Department of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Korea.
College of Korean Medicine, Gachon University, Seongnam 13120, Korea.
Int J Mol Sci. 2018 Nov 20;19(11):3660. doi: 10.3390/ijms19113660.
Shiunko ointment is composed of five ingredients including Lithospermi Radix (LR), Angelicae Gigantis Radix, sesame seed oil, beeswax, and swine oil. It is externally applied as a treatment for a wide range of skin conditions such as eczema, psoriasis, hair loss, burns, topical wounds, and atopic dermatitis. Deoxyshikonin is the major angiogenic compound extracted from LR. In this study, we investigated the efficacy of LR extract and deoxyshikonin on impaired wound healing in streptozotocin (STZ)-induced diabetic mice. Treatment with LR extract elevated tube formation in human umbilical vein endothelial cells (HUVECs) and exerted antioxidant activity. An open skin wound was produced on the backs of diabetic mice and was then topically treated with deoxyshikonin or vehicle. In addition, deoxyshikonin promoted tube formation in high glucose conditions exposed to HUVECs, and which may be regulated by increased VEGFR2 expression and phosphorylation of Akt and p38. Our results demonstrate that deoxyshikonin application promoted wound repair in STZ-induced diabetic mice. Collectively, these data suggest that deoxyshikonin is an active ingredient of LR, thereby contributing to wound healing in patients with diabetes.
麝香壮骨膏由五味成分组成，包括紫草、独活、芝麻油、蜂蜡和猪油。它被用作治疗各种皮肤疾病的外用药物，如湿疹、银屑病、脱发、烧伤、创面和特应性皮炎。去氧紫草素是从紫草中提取的主要血管生成化合物。在这项研究中，我们研究了紫草提取物和去氧紫草素对链脲佐菌素（STZ）诱导的糖尿病小鼠受损伤口愈合的疗效。紫草提取物可提高人脐静脉内皮细胞（HUVEC）的管形成能力，并发挥抗氧化活性。在糖尿病小鼠的背部制造开放性皮肤伤口，然后用去氧紫草素或载体进行局部治疗。此外，去氧紫草素促进了在高葡萄糖条件下暴露于 HUVEC 的管形成，这可能是通过增加 VEGFR2 表达和 Akt 和 p38 的磷酸化来调节的。我们的结果表明，去氧紫草素的应用促进了 STZ 诱导的糖尿病小鼠的伤口修复。总的来说，这些数据表明，去氧紫草素是紫草的一种有效成分，从而有助于糖尿病患者的伤口愈合。
