Pu Shaoxia, Usuda Kento, Nagaoka Kentaro, Watanabe Gen
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
J Vet Med Sci. 2019 Jan 8;81(1):77-83. doi: 10.1292/jvms.18-0615. Epub 2018 Nov 22.
High temperature induces various metabolic disturbances in animals. However, no comprehensive information is currently available on the metabolic pathway affected by high environmental temperature. The present study examined metabolite content in the serum of heat challenged quails using metabolomic analysis. In the present study, female quails with normal laying rate at 20 weeks kept in standard condition (control group) or exposed to 34°C 4 hr per day (12:00 to 16:00 hr)(heat group) for 10 consecutive days. The metabolomic analysis identified 165 metabolites in the serum, and significant differences were observed in the serum for 7 metabolites between two groups. An analysis by MetaboAnalyst, a web-based metabolome data tool, indicate that high temperature affect ketone body metabolism, butyrate metabolism, arginine and proline metabolism. Furthermore, histological examination of liver indicates a heat challenge induced abnormal lipid metabolism. Triglyceride and cholesterol level in the liver increased, however cholesterol level decreased in the serum. Genes related to lipid metabolism significantly increased in the liver after heat challenge. The present study demonstrated that high temperature cause liver damage, thus lipid metabolic was affected. Protect liver under high temperature could be one solution for coping with high temperatures in summer.
高温会在动物体内引发各种代谢紊乱。然而，目前尚无关于受高环境温度影响的代谢途径的全面信息。本研究采用代谢组学分析方法检测了热应激鹌鹑血清中的代谢物含量。在本研究中，将20周龄产蛋率正常的雌性鹌鹑饲养在标准条件下（对照组），或连续10天每天暴露于34°C环境4小时（12:00至16:00时）（热应激组）。代谢组学分析在血清中鉴定出165种代谢物，两组之间的7种血清代谢物存在显著差异。基于网络的代谢组数据工具MetaboAnalyst的分析表明，高温会影响酮体代谢、丁酸盐代谢、精氨酸和脯氨酸代谢。此外，肝脏组织学检查表明热应激会导致异常的脂质代谢。肝脏中的甘油三酯和胆固醇水平升高，但血清中的胆固醇水平降低。热应激后，肝脏中与脂质代谢相关的基因显著增加。本研究表明，高温会导致肝脏损伤，从而影响脂质代谢。在高温环境下保护肝脏可能是应对夏季高温的一种解决办法。
