大肠杆菌对2,3-二氨基萘进行亚硝化的机制：酶促产生一氧化氮，随后进行依赖氧气的化学亚硝化。
Mechanism for nitrosation of 2,3-diaminonaphthalene by Escherichia coli: enzymatic production of NO followed by O2-dependent chemical nitrosation.
作者信息
Ji X B, Hollocher T C
机构信息
Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254.
出版信息
Appl Environ Microbiol. 1988 Jul;54(7):1791-4. doi: 10.1128/aem.54.7.1791-1794.1988.
Abstract
The mechanism by which Escherichia coli can catalyze the nitrite-dependent nitrosation of 2,3-diaminonaphthalene (DAN), with formation of the corresponding fluorescent triazole, was studied. The reaction was dependent on production of a gaseous compound which can nitrosylate DAN upon contact with air. This compound was identified as nitric oxide (NO), and the kinetics of NO and triazole production are reported. NO and triazole were produced proportionally in a stoichiometric ratio, NO/triazole, of 1.4 to 1.7. Given the requirement for air, nitrosation of DAN probably proceeds via formation of the well-known strong nitrosylating agents N2O3 and N2O4 from NO. The parallel inhibition of NO and triazole production by azide and nitrate served to reinforce the link between nitrosation and nitrate reductase that had been established previously by others on genetic grounds.
摘要
研究了大肠杆菌催化2,3-二氨基萘（DAN）亚硝酸盐依赖性亚硝化反应并形成相应荧光三唑的机制。该反应依赖于一种气态化合物的产生，该化合物与空气接触时可使DAN亚硝化。该化合物被鉴定为一氧化氮（NO），并报道了NO和三唑产生的动力学。NO和三唑按化学计量比以1.4至1.7的比例成比例产生。鉴于对空气的需求，DAN的亚硝化可能通过由NO形成众所周知的强亚硝化剂N2O3和N2O4来进行。叠氮化物和硝酸盐对NO和三唑产生的平行抑制作用加强了先前其他人基于遗传学建立的亚硝化与硝酸盐还原酶之间的联系。
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