German Federal Institute for Risk Assessment, Berlin, Germany.
Landesbetrieb Hessisches Landeslabor, Kassel, Germany.
Food Environ Virol. 2019 Mar;11(1):1-8. doi: 10.1007/s12560-018-9360-6. Epub 2018 Nov 21.
Increasing numbers of hepatitis E cases are currently recognized in many European countries. The zoonotic hepatitis E virus (HEV) genotype 3 mainly circulates in domestic pigs and wild boars, and can be transmitted to humans via consumption of insufficiently heated meat or meat products produced from those animals. Here, a detailed protocol for detection of HEV RNA in meat products is provided, which is based on the method originally described by Szabo et al. (Intl J Food Microbiol 215:149-156, 2015). It consists of a TRI Reagent®/chloroform-based food matrix homogenization, a silica bead-based RNA extraction and a real-time RT-PCR-based RNA detection. The method was further validated in a ring trial with nine independent laboratories using pork liver sausage samples artificially contaminated with different amounts of HEV. The results indicate sufficient sensitivity, specificity, and accuracy of the method for its broad future use in survey studies, routine food control or outbreak investigations.
目前，许多欧洲国家都发现了越来越多的戊型肝炎病例。人畜共患的戊型肝炎病毒（HEV）基因型 3 主要在猪和野猪中传播，人类可通过食用未充分加热的肉类或用这些动物生产的肉类制品而感染该病毒。本研究提供了一种详细的检测肉类产品中 HEV RNA 的方法，该方法基于 Szabo 等人最初描述的方法（Intl J Food Microbiol 215:149-156, 2015）。它包括基于 TRI Reagent®/氯仿的食品基质匀浆化、基于硅珠的 RNA 提取和基于实时 RT-PCR 的 RNA 检测。该方法已在使用不同量 HEV 人工污染的猪肝香肠样本的九家独立实验室的环试验中得到进一步验证。结果表明，该方法具有足够的灵敏度、特异性和准确性，可广泛用于调查研究、常规食品控制或暴发调查。
