Risk Assessment Division, Federal Food Safety and Veterinary Office FSVO, Schwarzenburgstrasse 155, Bern, 3003, Switzerland.
Food Environ Virol. 2018 Sep;10(3):263-271. doi: 10.1007/s12560-018-9340-x. Epub 2018 Feb 28.
Seroprevalence data for pig herds suggested that there must be a relevant reservoir for hepatitis E virus (HEV) in Switzerland. To know more about the viral presence in ready-to-eat meat products, we screened pork liver sausages and raw meat sausages from the Swiss retail market for the presence of HEV. Testing was performed with a detection method where the virus extraction step was optimized. As for the performance of the improved method, the mean recovery rate for the mengovirus process control was 24.4%, whereas for HEV-inoculated sample matrices between 10.4 and 100% were achieved. The limit of detection was about 1.56 × 10 and 1.56 × 10 genome copies per gram for liver sausages and raw meat sausages, respectively. In the screening programme, HEV-RNA was detected in 10 of total 90 (11.1%) meat products, 7 of 37 (18.9%) liver sausages, and 3 of 53 (5.7%) raw meat sausages. Virus loads of up to 5.54 log HEV genome copies per gram were measured. All sequences retrieved from positive samples belonged to HEV genotype 3. The significance of the presented work was a current overview of the HEV prevalence in ready-to-eat meat products on the Swiss retail marked and an improvement of the extraction efficiency of the HEV detection method.
血清流行数据表明，瑞士猪群中必然存在戊型肝炎病毒（HEV）的相关储存库。为了更多地了解即食肉类产品中病毒的存在情况，我们对瑞士零售市场的猪肝香肠和生肉香肠进行了 HEV 检测。测试采用了一种检测方法，优化了病毒提取步骤。就改进方法的性能而言，在用于控制病毒的猪轮状病毒检测中，平均回收率为 24.4%，而接种 HEV 的样本基质的回收率在 10.4%至 100%之间。对于肝香肠和生肉香肠，检测限分别约为 1.56×10 和 1.56×10 基因组拷贝/克。在筛查计划中，在总共 90 份（11.1%）肉类产品中的 10 份、37 份肝香肠中的 7 份和 53 份生肉香肠中的 3 份中检测到了 HEV-RNA。测量到的病毒载量高达 5.54 log HEV 基因组拷贝/克。从阳性样本中获得的所有序列均属于 HEV 基因型 3。本工作的意义在于，目前对瑞士零售市场即食肉类产品中 HEV 流行率的概述，以及改进 HEV 检测方法的提取效率。
