Department of Gynecology and Obstetrics, Faculty of Health Sciences, Wroclaw Medical University, Poland.
Department of Molecular and Cellular Biology, Faculty of Pharmacy with Division of Laboratory Diagnostics, Wroclaw Medical University, Poland.
Adv Clin Exp Med. 2019 Feb;28(2):223-228. doi: 10.17219/acem/87045.
Vaginitis is one of the most common problems in clinical medicine and is cited most often during visits to obstetricians and gynecologists. Most of the inflammation cases are caused by candidiasis trichomoniasis and bacterial vaginosis. Therefore, treatment of vaginal infections must use antibiotic or antifungal drugs, which often provide quick relief to the patient. The real cause of the problem - disrupting the ecosystem of the vagina - remains unchanged. Thus, new therapeutic compounds are being explored.
The aim of our study was to evaluate the effect of a natural substance: tamanu oil, an extract from the plant Calophyllum inophyllum, applied to the human fibroblast cell line (normal human dermal fibroblasts - NHDFs) and to the isolated human fibroblasts from the vagina (human vaginal fibroblasts - HVFs) in vitro.
We evaluated the viability of cells by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay after incubation only with tamanu oil and with electroporation (EP). We also examined the immunocytochemical reaction of collagen type III and mitochondrial superoxide dismutase (MnSOD) under established conditions.
Tamanu oil increased the proliferation of cells and the amount of collagen III. It has been shown that the C. inophyllum extract stimulates the proliferation of commercial fibroblasts. For direct application in patients, one should use C. inophyllum extract in the range of 1:10-1:100 (saline dilution).
The use of this extract (at concentrations indicated by the studies presented here) stimulates the healing processes (increased expression of collagen type III), and has anti-inflammatory, analgesic and antiseptic qualities.
阴道炎是临床医学中最常见的问题之一，也是妇产科就诊最常提到的问题。大多数炎症是由念珠菌病、滴虫病和细菌性阴道病引起的。因此，阴道感染的治疗必须使用抗生素或抗真菌药物，这通常能为患者迅速缓解症状。但问题的真正原因——阴道生态系统的破坏——仍然没有改变。因此，人们正在探索新的治疗化合物。
我们的研究目的是评估一种天然物质——马鲁拉油（来自植物千层塔的提取物）对人成纤维细胞系（正常人类真皮成纤维细胞-NHDFs）和体外分离的人阴道成纤维细胞（人阴道成纤维细胞-HVF）的影响。
我们通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）试验评估仅用马鲁拉油和电穿孔（EP）孵育后细胞的活力。我们还在既定条件下检查了胶原 III 型和线粒体超氧化物歧化酶（MnSOD）的免疫细胞化学反应。
马鲁拉油增加了细胞的增殖和 III 型胶原的含量。研究表明，千层塔提取物刺激商业成纤维细胞的增殖。为了直接在患者中应用，应该将 C. inophyllum 提取物在 1:10-1:100（盐水稀释）的范围内使用。
使用这种提取物（如本文所示的研究中所指示的浓度）可刺激愈合过程（增加 III 型胶原的表达），并具有抗炎、镇痛和防腐的特性。
