The Procter and Gamble Company, Research and Development, Mason Business Center, Cincinnati, OH 45040, USA.
The Procter and Gamble Company, Research and Development, Sharon Woods Technical Center, Cincinnati, OH 45241, USA.
Biochim Biophys Acta Biomembr. 2019 Feb 1;1861(2):403-409. doi: 10.1016/j.bbamem.2018.11.007. Epub 2018 Nov 19.
The superficial layer of the skin, the stratum corneum (SC), consists of corneocytes surrounded by lipid regions and acts as a protective barrier for the body against water loss, toxic agents and microorganisms. As most substances permeate the stratum corneum through the lipid regions, lipid organization is considered crucial for the skin barrier function. Here, we investigate the potential of in vivo confocal Raman spectroscopy to describe the composition and organization of the SC. Confocal Raman spectroscopy is finding increasing use in the characterization of skin in biomedical, pharmaceutical and cosmetic applications. In this work, we analyze the spectra using chemometric methods and obtain principal components that correspond to the primary skin constituents: protein (keratin), natural moisturizing factor (NMF), water and lipid contributions in both ordered (orthorhombic) and disordered structural organization. By identifying these important components of the SC, these results highlight the utility of this in vivo, non-invasive, and depth resolved tool at the forefront of skin research.
皮肤的表层，即角质层（SC），由角质细胞组成，周围是脂质区域，作为身体防止水分流失、有毒物质和微生物的保护屏障。由于大多数物质通过脂质区域渗透到角质层，因此脂质组织被认为对皮肤屏障功能至关重要。在这里，我们研究了体内共聚焦拉曼光谱在描述 SC 组成和组织中的潜在应用。共聚焦拉曼光谱在生物医学、制药和化妆品应用中对皮肤的表征越来越多。在这项工作中，我们使用化学计量方法分析光谱，并获得与主要皮肤成分相对应的主成分：蛋白质（角蛋白）、天然保湿因子（NMF）、水和脂质在有序（正交）和无序结构组织中的贡献。通过鉴定 SC 的这些重要成分，这些结果突出了这种处于皮肤研究前沿的体内、非侵入性和深度分辨工具的实用性。
