University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstr. 30, 48149, Münster, Germany; Elemental Bioimaging Facility, University of Technology Sydney, Broadway, 2007, NSW, Australia.
University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstr. 30, 48149, Münster, Germany.
J Trace Elem Med Biol. 2019 Jan;51:212-218. doi: 10.1016/j.jtemb.2018.10.021. Epub 2018 Nov 1.
A novel analytical method to detect the retention of gadolinium from contrast agents for magnetic resonance imaging (MRI) in tissue samples of patients is presented. It is based on laser ablation - inductively coupled plasma - triple quadrupole - mass spectrometry (LA-ICP-MS/MS). Both Gd and P were monitored with a mass shift of +16, corresponding to mono-oxygenated species, as well as Zn, Ca, and Fe on-mass. This method resulted in a significantly reduced background and improved limits of detection not only for phosphorus, but also for gadolinium. These improvements were essential to perform elemental bioimaging with improved resolution of 5 μm x 5 μm, allowing the detection of small Gd deposits in fibrotic skin and brain tumour tissue with diameters of approximately 50 μm. Detailed analyses of these regions revealed that most Gd was accompanied with P and Ca, indicating co-precipitation.
介绍了一种用于检测磁共振成像（MRI）造影剂中钆在患者组织样本中滞留的新分析方法。它基于激光烧蚀 - 电感耦合等离子体 - 三重四极杆 - 质谱法（LA-ICP-MS/MS）。通过质量位移 +16 同时监测 Gd 和 P，对应于单氧化物种，以及 Zn、Ca 和 Fe 的质量。这种方法不仅显著降低了背景，而且还提高了磷以及钆的检测限。这些改进对于进行元素生物成像至关重要，分辨率提高到 5μm x 5μm，允许检测直径约为 50μm 的纤维化皮肤和脑肿瘤组织中的小 Gd 沉积物。对这些区域的详细分析表明，大多数 Gd 与 P 和 Ca 一起存在，表明共沉淀。
