Wong J H, Aguero B, Gupta R K, Morton D L
Department of Surgery, John Wayne Cancer Clinic, Jonsson Comprehensive Cancer Center, UCLA School of Medicine 90024.
Cancer Immunol Immunother. 1988;27(2):142-6. doi: 10.1007/BF00200019.
A well-characterized 69.5 x 10(3) dalton glycoprotein fetal antigen (FA), isolated from the spent culture medium of a melanoma cell line, UCLA-SO-14 (M14), was utilized to characterize the antigen component of circulating immune complexes (CIC) from melanoma patients. Ten serum samples from five patients with stage II melanoma at 1 and 4 months prior to the clinical detection of recurrent disease were selected for study. The CIC were dissociated with low pH and ultrafiltered through a 100 x 10(3) dalton exclusion limit membrane. The low pH treatment resulted in an increase in antibody titer in eight of ten serum samples. The antibody activity in membrane immunofluorescence was quantitatively inhibited by the filtered antigen fraction and purified FA, suggesting the presence of anti-FA antibodies in the treated serum, which possibly were complexed with FA in the untreated sample. As determined by competitive inhibition in an enzyme-linked immunosorbent assay, the filtrate (antigen fraction) contained an antigen that was immunologically similar to FA. These results clearly demonstrate that FA, expressed on the cell surface of melanoma cells, is present in CIC of selected melanoma patients.
从黑色素瘤细胞系UCLA - SO - 14（M14）的用过的培养基中分离出一种特性明确的69.5×10³道尔顿糖蛋白胎儿抗原（FA），用于鉴定黑色素瘤患者循环免疫复合物（CIC）的抗原成分。选取了5例II期黑色素瘤患者在复发疾病临床检测前1个月和4个月时的10份血清样本进行研究。用低pH值解离CIC，并通过100×10³道尔顿截留分子量的膜进行超滤。低pH值处理使10份血清样本中的8份抗体滴度升高。膜免疫荧光中的抗体活性被过滤后的抗原组分和纯化的FA定量抑制，表明处理后的血清中存在抗FA抗体，这些抗体在未处理的样本中可能与FA形成了复合物。通过酶联免疫吸附测定中的竞争抑制确定，滤液（抗原组分）含有一种在免疫上与FA相似的抗原。这些结果清楚地表明，黑色素瘤细胞表面表达的FA存在于所选黑色素瘤患者的CIC中。
