Xu Lei, Shen Zhi-Lin, Chen Wen, Si Guo-Yang, Meng Yan, Guo Ning, Sun Xu, Cai Yong-Ping, Lin Yi, Gao Jun-Shan
School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Mol Biol Rep. 2019 Feb;46(1):161-175. doi: 10.1007/s11033-018-4457-4. Epub 2018 Nov 22.
The multidrug and toxic compound extrusion (MATE) protein belongs to a secondary transporter family, which plays a role in transporting different kinds of substrates like phytohormones and secondary metabolites. In plant, MATE transporters related to the endogenous and exogenous mechanisms of detoxification for secondary metabolites such as alkaloids, flavonoids, anthocyanins and other secondary metabolites have been studied. However, a genome-wide analysis of the MATE family is rarely reported in upland cotton (Gossypium hirsutum L.). In the study, a total of 72 GhMATEs were identified from the genome of upland cotton, which were classified into four subfamilies with possible diverse functions such as transport of proanthocyanidins (PAs), accumulation of alkaloids, extrusion of xenobiotic compounds, regulation of disease resistance and response to abiotic stresses. Meanwhile, the gene structure, evolutionary relationship, physical location, conservative motifs, subcellular localization and gene expression pattern of GhMATEs have been further analysed. Three of these MATE genes (GhMATE12, GhMATE16 and GhMATE38) were identified as candidate genes due to their functions in transport of PA similar to GhTT12. These results provide a new perspective on upland cotton MATE gene family for their potential roles in transport of PA and a theoretical basis for further analyzing the function of MATE genes and improving the fiber quality of brown cotton.
多药与有毒化合物排出（MATE）蛋白属于次级转运蛋白家族，在运输多种底物（如植物激素和次生代谢产物）中发挥作用。在植物中，与生物碱、黄酮类化合物、花青素等次生代谢产物的内源性和外源性解毒机制相关的MATE转运蛋白已得到研究。然而，陆地棉（Gossypium hirsutum L.）中MATE家族的全基因组分析鲜有报道。在本研究中，从陆地棉基因组中总共鉴定出72个GhMATEs，它们被分为四个亚家族，可能具有不同的功能，如原花青素（PAs）运输、生物碱积累、异源生物化合物排出、抗病性调节和对非生物胁迫的响应。同时，对GhMATEs的基因结构、进化关系、物理位置、保守基序、亚细胞定位和基因表达模式进行了进一步分析。其中三个MATE基因（GhMATE12、GhMATE16和GhMATE38）因其在PA运输中与GhTT12相似的功能而被鉴定为候选基因。这些结果为陆地棉MATE基因家族在PA运输中的潜在作用提供了新的视角，并为进一步分析MATE基因的功能和改善棕色棉纤维品质提供了理论基础。
