State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China.
Int J Mol Sci. 2024 Mar 29;25(7):3859. doi: 10.3390/ijms25073859.
The multidrug and toxin efflux (MATE) family participates in numerous biological processes and plays important roles in abiotic stress responses. However, information about the family genes in remains unclear. In this study, our genome-wide investigation identified ninety genes in , which were divided into five evolutionary clades. family members are located on eleven chromosomes, and a total of thirty exist in tandem duplication. The promoter analysis showed that most contain the cis-regulatory elements associated with stress and hormonal responses. In addition, we discovered that most genes responded to abiotic stresses (aluminum, drought, high temperatures, and low temperatures). Weighted correlation network analysis showed that 147 candidate transcription factor genes regulated the expression of 14 genes, and it was verified through a double-luciferase assay. Overall, our findings offer valuable information for the characterization of the gene mechanism in responding to abiotic stress and exhibit promising prospects for the stress tolerance breeding of .
多药和毒素外排（MATE）家族参与众多生物过程，并在非生物胁迫反应中发挥重要作用。然而，关于 的家族基因的信息尚不清楚。在这项研究中，我们进行了全基因组研究，在 中鉴定出了 90 个基因，这些基因分为五个进化枝。 家族成员位于十一条染色体上，共有三十个串联重复。启动子分析表明，大多数 含有与应激和激素反应相关的顺式调控元件。此外，我们发现大多数 基因对非生物胁迫（铝、干旱、高温和低温）有响应。加权相关网络分析显示，147 个候选转录因子基因调控 14 个基因的表达，通过双荧光素酶报告基因检测得到了验证。总的来说，我们的研究结果为研究 基因在应对非生物胁迫中的作用机制提供了有价值的信息，并为 的抗胁迫育种展示了广阔的前景。
