Shao Dongnan, Zhu Qian-Hao, Liang Qian, Wang Xuefeng, Li Yanjun, Sun Yuqiang, Zhang Xinyu, Liu Feng, Xue Fei, Sun Jie
Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi, China.
CSIRO Agriculture and Food, Canberra, ACT, Australia.
Front Plant Sci. 2022 Mar 31;13:788828. doi: 10.3389/fpls.2022.788828. eCollection 2022.
Many factors, including illumination, affect anthocyanin biosynthesis and accumulation in plants. light quality is the key factor affecting the process of photoinduced anthocyanin biosynthesis and accumulation. We observed that the red color of the Upland cotton accession Huiyuan with the mutation turned to normal green color under light-emitting diodes (LEDs), which inspired us to investigate the effect of red and blue lights on the biosynthesis and accumulation of anthocyanins. We found that both red and blue lights elevated accumulation of anthocyanins. Comparative transcriptomic analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and GSEA, revealed that genes differentially expressed under different light conditions were enriched with the pathways of circadian rhythm, phenylpropanoid biosynthesis, anthocyanin biosynthesis, and flavone and flavonol biosynthesis. Not surprisingly, all the major structural genes related to biosynthesis of anthocyanins, including the key regulatory MYB transcription factor () and anthocyanin transporter (), were induced by red or blue light treatment. However, and related to biosynthesis of proanthocyanidins were more significantly up-regulated by red light radiation than by blue light radiation. Vice versa, the accumulation of anthocyanins under red light was not as high as that under blue light. In addition, we demonstrated a potential role of , a key regulator in plant circadian rhythms, in regulation of anthocyanin accumulation, which could be achieved via interaction with . Together, these results indicate different effect of red and blue lights on biosynthesis and accumulation of anthocyanins and a potential module including and in regulation of anthocyanin accumulation in cotton. These results also suggest that the substrates responsible the synthesis of anthocyanins under blue light is diverted to biosynthesis of proanthocyanidin under red light.
包括光照在内的许多因素都会影响植物中花青素的生物合成和积累。光质是影响光诱导花青素生物合成和积累过程的关键因素。我们观察到，具有该突变的陆地棉品种汇源的红色在发光二极管（LED）光照下变为正常绿色，这启发我们研究红光和蓝光对花青素生物合成和积累的影响。我们发现红光和蓝光都能提高花青素的积累。包括基因本体论（GO）、京都基因与基因组百科全书（KEGG）和基因集富集分析（GSEA）在内的比较转录组分析表明，在不同光照条件下差异表达的基因在昼夜节律、苯丙烷类生物合成、花青素生物合成以及黄酮和黄酮醇生物合成途径中富集。不出所料，所有与花青素生物合成相关的主要结构基因，包括关键调控MYB转录因子（）和花青素转运蛋白（），都受到红光或蓝光处理的诱导。然而，与原花青素生物合成相关的和在红光照射下比蓝光照射下上调更为显著。反之，红光下花青素的积累不如蓝光下高。此外，我们证明了植物昼夜节律中的关键调节因子在花青素积累调控中的潜在作用，这可以通过与相互作用来实现。总之，这些结果表明红光和蓝光对花青素生物合成和积累有不同影响，并且在棉花花青素积累调控中存在一个包括和的潜在模块。这些结果还表明，蓝光下负责花青素合成的底物在红光下被转移到原花青素的生物合成中。
