Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang 524025, China.
Int J Mol Sci. 2018 Nov 22;19(12):3700. doi: 10.3390/ijms19123700.
The paired-box 3 () is a transcription factor and it plays an important part in melanin synthesis. In this study, a new gene was identified from (, ) () by RACE-PCR (rapid-amplification of cDNA ends-polymerase chain reaction) and its effect on melanin synthesis was deliberated by RNA interference (RNAi). The cDNA of was 2250 bp long, containing an open reading fragment of 1365 bp encoding 455 amino acids. Amino acid alignment and phylogenetic tree showed shared the highest (69.2%) identity with of . Tissue expression profile showed that had the highest expression in mantle, a nacre-formation related tissue. The silencing significantly inhibited the expression of , , and , genes involved in -mediated melanin synthesis, but had no effect on and an increase effect on . Furthermore, the knockdown obviously decreased the tyrosinase activity, the total content of eumelanin and the proportion of PDCA (pyrrole-2,3-dicarboxylic acid) in eumelanin, consistent with influence of tyrosinase () knockdown. These data indicated that played an important regulating role in melanin synthesis by pathway in .
配对盒 3 () 是一种转录因子，它在黑色素合成中起着重要作用。在这项研究中，我们通过 RACE-PCR（快速扩增 cDNA 末端聚合酶链反应）从贻贝 () () 中鉴定出一个新的 基因，并通过 RNA 干扰 (RNAi) 研究其对黑色素合成的影响。 的 cDNA 长 2250bp，包含一个编码 455 个氨基酸的 1365bp 的开放阅读框。氨基酸比对和系统发育树显示，与 的相似度最高（69.2%）。组织表达谱显示，在与珍珠层形成相关的组织中， 具有最高的表达水平。 沉默显著抑制了黑色素合成相关的 、 、 和 基因的表达，但对 和 没有影响，并增加了 的表达。此外， 敲低明显降低了酪氨酸酶活性、真黑色素的总含量和真黑色素中 PDCA（吡咯-2,3-二羧酸）的比例，这与酪氨酸酶 () 敲低的影响一致。这些数据表明， 在 中通过 途径在黑色素合成中发挥重要的调节作用。
