College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China.
Genes (Basel). 2022 Aug 4;13(8):1391. doi: 10.3390/genes13081391.
Shin color diversity is a widespread phenomenon in birds. In this study, ducks were assessed to identify candidate genes for yellow, black, and spotted tibiae. For this purpose, we performed whole-genome resequencing of an F population consisting of 275 ducks crossed between Runzhou crested-white ducks and Cherry Valley ducks. We obtained 12.6 Mb of single nucleotide polymorphism (SNP) data, and the three shin colors were subsequently genotyped. Genome-wide association studies (GWASs) were performed to identify candidate and potential SNPs for the three shin colors. According to the results, 2947 and 3451 significant SNPs were associated with black and yellow shins, respectively, and six potential SNPs were associated with spotted shins. Based on the SNP annotations, the , , POU family members, and the SLC superfamily were the candidate genes regulating pigmentation. In addition, the isoforms of , , , and MITF-M were significantly different between the black and yellow tibiae. and may have synergistic roles in the regulation of melanin synthesis, and their mutations may lead to phenotypic differences in the melanin deposition between individuals. This study provides new insights into the genetic factors that may influence tibia color diversity in birds.
鸟类的足部颜色多样性是一种广泛存在的现象。在这项研究中，我们评估了鸭子，以鉴定黄色、黑色和斑点状胫骨的候选基因。为此，我们对由 275 只鸭子组成的 F1 群体进行了全基因组重测序，这些鸭子是由 RUNZHOU 冠白鸭和樱桃谷鸭杂交而成。我们获得了 12.6 Mb 的单核苷酸多态性（SNP）数据，随后对这三种胫骨颜色进行了基因型分析。我们进行了全基因组关联研究（GWAS），以鉴定与这三种胫骨颜色相关的候选基因和潜在 SNP。结果表明，2947 个和 3451 个显著 SNP 分别与黑色和黄色胫骨相关，6 个潜在 SNP 与斑点状胫骨相关。根据 SNP 注释，、、POU 家族成员和 SLC 超家族是调节色素沉着的候选基因。此外，、、、和 MITF-M 的异构体在黑色和黄色胫骨之间存在显著差异。和可能在黑色素合成的调控中具有协同作用，它们的突变可能导致个体之间黑色素沉积的表型差异。这项研究为影响鸟类胫骨颜色多样性的遗传因素提供了新的见解。
