Department of Periodontics, School of Stomatology, China Medical University, No. 117 Nanjing North Street, Shenyang, 110002, China.
Department of Oral Biology, School of Stomatology, China Medical University, No. 117 Nanjing North Street, Shenyang, 110002, China.
Appl Microbiol Biotechnol. 2019 Feb;103(3):1393-1404. doi: 10.1007/s00253-018-9475-6. Epub 2018 Nov 23.
Mounting evidence suggests a causal relationship between specific bacterial infections or microbial compositions and the development of certain malignant neoplasms. In this study, we performed research through 16S rRNA amplicon sequencing, qPCR and fluorescence in situ hybridization to certify the relationship between periodontal pathogens and oral squamous cell carcinoma (OSCC). Subgingival plaque, cancer and paracancerous tissues from 6 patients with OSCC were selected for mapping bacterial profiles by 16S rRNA amplicon sequencing. The research showed that periodontal pathogens were enriched in cancer and paracancerous tissues, while the bacterial profiles were similar between the cancer tissues and subgingival plaque. Furthermore, the relative abundance of Porphyromonas gingivalis, Fusobacterium nucleatum and Streptococcus sanguinis was detected in 61 cancer tissues, paracancerous tissues and subgingival plaque samples and in 30 normal tissues by qPCR. The results revealed that P. gingivalis and F. nucleatum existed at higher levels in cancer tissue than in normal tissues and were correlated with subgingival plaques. P. gingivalis was detected using a special oligonucleotide probe in 60.7% of OSCC tissues, 32.8% of paracancerous tissues and 13.3% of normal tissues. Relevance analysis showed that P. gingivalis infection was positively associated with late clinical staging, low differentiation and lymph node metastasis in patients with OSCC, which was accompanied by deeper periodontal pockets, severe clinical attachment loss and loss of teeth. This study revealed that there might be a close relationship between oral microorganisms, particularly periodontal pathogens, and OSCC, which might enrich the pathogenesis of oral squamous carcinoma.
越来越多的证据表明，特定的细菌感染或微生物组成与某些恶性肿瘤的发展之间存在因果关系。在这项研究中，我们通过 16S rRNA 扩增子测序、qPCR 和荧光原位杂交来研究牙周病原体与口腔鳞状细胞癌 (OSCC) 之间的关系。我们从 6 名 OSCC 患者中选择了龈下菌斑、癌症和癌旁组织，通过 16S rRNA 扩增子测序绘制细菌图谱。研究表明，牙周病原体在癌症和癌旁组织中富集，而癌症组织和龈下菌斑的细菌谱相似。此外，通过 qPCR 检测了 61 个癌症组织、癌旁组织和龈下菌斑样本以及 30 个正常组织中的牙龈卟啉单胞菌、核梭杆菌和链球菌的相对丰度。结果表明，牙龈卟啉单胞菌和核梭杆菌在癌症组织中的丰度高于正常组织，并且与龈下菌斑相关。在 60.7%的 OSCC 组织、32.8%的癌旁组织和 13.3%的正常组织中使用特殊的寡核苷酸探针检测到了牙龈卟啉单胞菌。相关性分析表明，牙龈卟啉单胞菌感染与 OSCC 患者的晚期临床分期、低分化和淋巴结转移呈正相关，且与牙周袋较深、临床附着丧失严重和牙齿缺失有关。这项研究表明，口腔微生物，特别是牙周病原体，与 OSCC 之间可能存在密切关系，这可能丰富口腔鳞状癌的发病机制。
