牙龈卟啉单胞菌外膜囊泡增强口腔鳞状细胞癌细胞的增殖和转移。

Porphyromonas gingivalis outer membrane vesicles augments proliferation and metastasis of oral squamous cell carcinoma cells.

作者信息

Zeng Yanru, Wang Yiyang, Shi Xiaona, Zhao Yuanhao, Tang Yue, Liu Shanshan, Zhu Xiaofeng

机构信息

Department of Oral Maxillo-Facial Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China.

Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.

出版信息

BMC Oral Health. 2025 May 10;25(1):701. doi: 10.1186/s12903-025-05937-z.

DOI:10.1186/s12903-025-05937-z

PMID:40348995

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12065146/

Abstract

BACKGROUND

Porphyromonas gingivalis (P. gingivalis) is closely related to Oral squamous cell carcinoma (OSCC), and P. gingivalis outer membrane vesicles (OMVs) is the main pathogenic factor, which is associated with periodontitis, atherosclerosis and other diseases. However, few studies have reported an association between P. gingivalis OMVs and OSCC. The purpose of this study was to establish the clinical relationship between P. gingivalis and OSCC based on clinical samples. Further, the effect of P. gingivalis OMVs on OSCC was observed with cell model in vitro, and the possible molecular mechanism was discussed.

METHODS

Immunohistochemistry was used to detect the abundance of P. gingivalis in OSCC and its paired paracancer tissues, and to analyze the correlation between P. gingivalis and clinicopathological parameters of patients. P. gingivalis OMVs were isolated to observe its effects on the proliferation and migration of OSCC cell lines. RNA-seq was performed and the expression of differentially expressed genes (DEGs) was detected by real-time quantitative PCR (RT-qPCR) to explore the potential mechnism of P. gingivalis OMVs on OSCC progression.

RESULTS

The abundance of P. gingivalis in OSCC was higher than that in para-cancerous tissues, and was positively correlated with the degree of tissue differentiation (P = 0.028), T stage (P < 0.001), and clinical stage (P = 0.011). P. gingivalis OMVs promoted the proliferation and migration of HN6 cells, and promoted the proliferation of CAL27 cells, but had no significant effect on its migration. P. gingivalis OMVs treatment attenuated the expressions of TNFSF15, ZNF292, ATRX, ASPM and KIF20B in CAL27 and HN6 cells.

CONCLUSION

This study suggests that P. gingivalis may be an indicator of poor prognosis for OSCC. P. gingivalis OMVs may down-regulate the expression of TNFSF15, ZNF292, ATRX, ASPM, KIF20B and participate in the occurrence and development of OSCC.

摘要

背景

牙龈卟啉单胞菌（P. gingivalis）与口腔鳞状细胞癌（OSCC）密切相关，牙龈卟啉单胞菌外膜囊泡（OMVs）是主要致病因素，与牙周炎、动脉粥样硬化等疾病相关。然而，很少有研究报道牙龈卟啉单胞菌OMVs与OSCC之间的关联。本研究旨在基于临床样本建立牙龈卟啉单胞菌与OSCC之间的临床关系。此外，通过体外细胞模型观察牙龈卟啉单胞菌OMVs对OSCC的影响，并探讨其可能的分子机制。

方法

采用免疫组织化学方法检测OSCC及其配对癌旁组织中牙龈卟啉单胞菌的丰度，并分析牙龈卟啉单胞菌与患者临床病理参数之间的相关性。分离牙龈卟啉单胞菌OMVs，观察其对OSCC细胞系增殖和迁移的影响。进行RNA测序，并通过实时定量PCR（RT-qPCR）检测差异表达基因（DEGs）的表达，以探讨牙龈卟啉单胞菌OMVs对OSCC进展的潜在机制。

结果

OSCC中牙龈卟啉单胞菌的丰度高于癌旁组织，且与组织分化程度（P = 0.028）、T分期（P < 0.001）和临床分期（P = 0.011）呈正相关。牙龈卟啉单胞菌OMVs促进了HN6细胞的增殖和迁移，并促进了CAL27细胞的增殖，但对其迁移无显著影响。牙龈卟啉单胞菌OMVs处理使CAL27和HN6细胞中TNFSF15、ZNF292、ATRX、ASPM和KIF20B的表达减弱。