假定的β-葡萄糖苷酶BGL3I调节纤维素酶的诱导。
The putative β-glucosidase BGL3I regulates cellulase induction in .
作者信息
Zou Gen, Jiang Yanping, Liu Rui, Zhu Zhihua, Zhou Zhihua
机构信息
1CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai, 200032 China.
Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agriculture Science, 1000 Jinqi Rd, Fengxian, 201403 Shanghai China.
出版信息
Biotechnol Biofuels. 2018 Nov 19;11:314. doi: 10.1186/s13068-018-1314-6. eCollection 2018.
BACKGROUND
The filamentous fungus (anamorph of ) displays increased cellulase expression while growing on inducers such as lactose or cellulose. However, the mechanism of cellulase induction in is not yet completely characterized. Here, a protein annotated as β-glucosidase (BGL3I) was found to be involved in cellulase induction in . The effects of BGL3I on cellulase production have not yet been fully understood.
RESULTS
Deletion of the gene had no influence on the growth of , but significantly increased its cellulase activities. Deletion of also resulted in decreased extracellular galactosidase activity, but significantly increased transcription of lactose permeases, which might be involved in lactose transport. Furthermore, deletion of enhanced the transcription levels of intracellular β-glucosidases and the regulator , which are all essential for lactose induction in . BGL3I was found to have a relatively high ability to hydrolyze sophorose, which is proposed to be the strongest natural inducer of cellulase synthesis in .
CONCLUSIONS
BGL3I may take part in the complex regulating system of cellulase induction. The deletion of offers a new strategy to improve strain performance.
背景
丝状真菌（的无性型）在乳糖或纤维素等诱导物上生长时，纤维素酶表达增加。然而，中纤维素酶诱导的机制尚未完全阐明。在此，发现一种注释为β-葡萄糖苷酶（BGL3I）的蛋白质参与了中的纤维素酶诱导。BGL3I对纤维素酶产生的影响尚未完全了解。
结果
基因的缺失对的生长没有影响，但显著提高了其纤维素酶活性。的缺失还导致细胞外半乳糖苷酶活性降低，但乳糖通透酶的转录显著增加，这可能与乳糖转运有关。此外，的缺失增强了细胞内β-葡萄糖苷酶和调节因子的转录水平，它们对中的乳糖诱导都是必不可少的。发现BGL3I具有相对较高的水解槐糖的能力，槐糖被认为是中纤维素酶合成最强的天然诱导物。
结论
BGL3I可能参与纤维素酶诱导的复杂调节系统。的缺失为提高菌株性能提供了一种新策略。
Zotero 插件