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黑曲霉细胞内β-葡萄糖苷酶BGL1B参与木质纤维素降解酶合成的调控。

Involvement of the intracellular β-glucosidase BGL1B from Aspergillus niger in the regulation of lignocellulose-degrading enzymes' synthesis.

作者信息

Zhang Zhen, Li Hua, Dong Feiyu, Lin Hui, Li Yanan, Cheng Kun, Chen Hongge

机构信息

College of Life Sciences, Henan Agricultural University, 218 Ping-an Ave., Zhengzhou, 450046, China.

出版信息

Biotechnol Biofuels Bioprod. 2025 Jan 27;18(1):11. doi: 10.1186/s13068-025-02610-z.

DOI:10.1186/s13068-025-02610-z
PMID:39871278
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11773763/
Abstract

BACKGROUND

Aspergillus niger is an important lignocellulose-degrading enzyme-producing strain. Multiple regulatory factors regulate the synthesis of lignocellulose-degrading enzymes in A. niger. We previously found that A. niger possessed an intracellular β-glucosidase BGL1B, and the intracellular localization of BGL1B and its active transglycosylation action prompted us to explore whether BGL1B was involved in the regulation of the synthesis of lignocellulose-degrading enzymes in A. niger.

RESULTS

In this study, by investigating the production of lignocellulose-degrading enzymes of bgl1B knockout strain (Δbgl1B) and overexpression strain (OE::bgl1B), it was found that BGL1B exhibited a repressive role on the expression of lignocellulose-degrading enzyme genes through carbon catabolite repression (CCR) way. On the other hand, BGL1B's transglycosylation products sophorose and laminaribiose were proved to be able to induce the expression of lignocellulose-degrading enzyme genes, which explained why OE::bgl1B showed the same enhanced enzyme activity and gene expression as Δbgl1B strain compared to the starting strain (WT).

CONCLUSIONS

The present study demonstrates that BGL1B plays dual regulatory roles in the regulation of the synthesis of lignocellulose-degrading enzymes in A. niger: the repressive role caused by BGL1B's hydrolysis product glucose and the induction role caused by BGL1B's transglycosylation products sophorose and laminaribiose. This study broadens the understanding of the regulatory network of the synthesis of lignocellulose-degrading enzymes in A. niger. Also, it provides a strategy to create an engineered strain with high production of lignocellulose-degrading enzymes.

摘要

背景

黑曲霉是一种重要的产木质纤维素降解酶菌株。多种调控因子调节黑曲霉中木质纤维素降解酶的合成。我们之前发现黑曲霉拥有一种细胞内β-葡萄糖苷酶BGL1B，BGL1B的细胞内定位及其活性转糖基化作用促使我们探究BGL1B是否参与黑曲霉中木质纤维素降解酶合成的调控。

结果

在本研究中，通过研究bgl1B基因敲除菌株（Δbgl1B）和过表达菌株（OE::bgl1B）的木质纤维素降解酶产量，发现BGL1B通过碳代谢物阻遏（CCR）方式对木质纤维素降解酶基因的表达发挥抑制作用。另一方面，已证明BGL1B的转糖基化产物槐糖和层二糖能够诱导木质纤维素降解酶基因的表达，这解释了为何与起始菌株（WT）相比，OE::bgl1B与Δbgl1B菌株表现出相同的酶活性增强和基因表达增强。

结论

本研究表明BGL1B在黑曲霉木质纤维素降解酶合成调控中发挥双重调控作用：BGL1B的水解产物葡萄糖引起的抑制作用以及BGL1B的转糖基化产物槐糖和层二糖引起的诱导作用。本研究拓宽了对黑曲霉木质纤维素降解酶合成调控网络的理解。此外，它为构建高产木质纤维素降解酶的工程菌株提供了一种策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/ee6b3c558859/13068_2025_2610_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/2da48a6dbe68/13068_2025_2610_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/44119691fb4e/13068_2025_2610_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/030f9ed68ad2/13068_2025_2610_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/145f3466d176/13068_2025_2610_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/1fe063f0cec8/13068_2025_2610_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/ee6b3c558859/13068_2025_2610_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/2da48a6dbe68/13068_2025_2610_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/44119691fb4e/13068_2025_2610_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/030f9ed68ad2/13068_2025_2610_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/145f3466d176/13068_2025_2610_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/1fe063f0cec8/13068_2025_2610_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb0/11773763/ee6b3c558859/13068_2025_2610_Fig6_HTML.jpg

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