Tyagi Akanksha, Shetty Jayaprakasha, Shetty Shriya, Kumar Basavarajappa Mohana, Shetty Alandur Veena, Nair Manju Raman
Department of Pedodontics and Preventive Dentistry, A B Shetty Memorial Institute of Dental Sciences, NITTE (Deemed to be University), Mangaluru, Karnataka, India.
Department of Centre for Stem Cell Research and Regenerative Medicine, K S Hegde Medical Academy, NITTE (Deemed to be University), Mangaluru, Karnataka, India.
Int J Clin Pediatr Dent. 2023 Nov;16(Suppl 3):240-246. doi: 10.5005/jp-journals-10005-2683.
Stem cells from human exfoliated deciduous teeth (SHED) provide an important autologous source for stem cell-based regenerative therapies with their easy acquisition and multipotency. However, the understanding of their antibacterial and immunomodulatory properties is limited. This research aims to determine whether SHED inhibits the growth of and , as well as whether or not it has immunomodulatory effects by measuring interleukins (ILs)-2 and -6 levels. SHEDs were derived from the pulp of deciduous teeth that had undergone up to two-thirds of their roots' resorption. Isolated SHEDs were characterized on their morphological features, viability, assessment of surface markers, and induction into osteocytes and adipocytes. SHED was tested for its antibacterial efficacy against and using a colony-forming units (CFU) assay. Lastly, we checked the cytokine levels by enzyme-linked immune sorbent assay (ELISA) for assessing the immunomodulatory properties of SHED. The results showed that the established SHED had fibroblastic morphology with higher viability. The ability to differentiate into osteocytes and adipocytes, as well as the expression of stem cell-specific markers, demonstrated their potential and flexibility under settings. SHED demonstrated antibacterial characteristics by significantly ( < 0.05) lowering CFU, whereas CFU was either unaffected by or just slightly affected by the cells. SHED also helped keep inflammatory indicators, including IL-2 and IL-6, at stable levels when compared to the control. The results indicate that SHED may aid in preventing or reducing an infection due to its antibacterial activity and may provide immunomodulatory activities by controlling the production of cytokines.
Tyagi A, Shetty J, Shetty S, Antibacterial and Immunomodulatory Properties of Stem Cells from Human Exfoliated Deciduous Teeth: An Study. Int J Clin Pediatr Dent 2023;16(S-3):S240-S246.
人脱落乳牙干细胞（SHED）易于获取且具有多能性，为基于干细胞的再生疗法提供了重要的自体来源。然而，对其抗菌和免疫调节特性的了解有限。本研究旨在通过测量白细胞介素（IL）-2和-6水平来确定SHED是否抑制变形链球菌和远缘链球菌的生长，以及它是否具有免疫调节作用。SHED来自牙根吸收达三分之二的乳牙牙髓。对分离出的SHED进行形态特征、活力、表面标志物评估以及向骨细胞和脂肪细胞诱导分化的鉴定。使用菌落形成单位（CFU）测定法检测SHED对变形链球菌和远缘链球菌的抗菌功效。最后，我们通过酶联免疫吸附测定（ELISA）检查细胞因子水平，以评估SHED的免疫调节特性。结果显示，所建立的SHED具有成纤维细胞形态且活力较高。在特定条件下，其向骨细胞和脂肪细胞分化的能力以及干细胞特异性标志物的表达证明了它们的潜能和灵活性。SHED通过显著降低变形链球菌CFU（P<0.05）表现出抗菌特性，而远缘链球菌CFU要么不受细胞影响，要么仅受到轻微影响。与对照组相比，SHED还有助于使包括IL-2和IL-6在内的炎症指标保持在稳定水平。结果表明，SHED可能因其抗菌活性有助于预防或减少感染，并可能通过控制细胞因子的产生提供免疫调节活性。
Tyagi A，Shetty J，Shetty S，人脱落乳牙干细胞的抗菌和免疫调节特性：一项体外研究。《国际临床儿科牙科学杂志》2023；16（S-3）：S240 - S246。
