利用新一代测序技术开发17个针对文殊兰属（石蒜科）的新型微卫星标记。
Development of 17 novel microsatellite markers for and (Amaryllidaceae) using next-generation sequencing.
作者信息
Chen I-Ju, Shii Chou-Tou, Chang Tsu-Liang, Hwu Kae-Kang
机构信息
Department of Horticulture and Landscape Architecture National Taiwan University No. 1, Sec. 4, Roosevelt Road Taipei 10617 Taiwan.
Department of Agronomy National Taiwan University No. 1, Sec. 4, Roosevelt Road Taipei 10617 Taiwan.
出版信息
Appl Plant Sci. 2018 Nov 14;6(11):e01198. doi: 10.1002/aps3.1198. eCollection 2018 Nov.
PREMISE OF THE STUDY
is an ornamental and medicinal plant. We developed microsatellite markers for and simultaneously by using a hybrid between these two species.
METHODS AND RESULTS
Ion Torrent next-generation sequencing produced 1,784,504 reads. Testing 64 primer sets allowed for the development of 17 novel microsatellite markers: 16 for , 10 for , and nine common markers. had one to 12 alleles per locus and observed and expected heterozygosity levels of 0-0.923 and 0.038-0.809, respectively. had three to 12 alleles per locus and observed and expected heterozygosity levels of 0-0.909 and 0.127-0.797, respectively. Ten markers were cross-amplified for .
CONCLUSIONS
Hybrid sequencing can facilitate the cost-effective development of molecular markers for parental species. The markers developed here are useful for studying population structure.
研究前提
[植物名称]是一种观赏兼药用植物。我们通过使用这两个物种之间的杂交种，同时为[植物名称1]和[植物名称2]开发了微卫星标记。
方法与结果
离子激流新一代测序产生了1,784,504条读数。对64对引物组进行测试后，开发出了17个新的微卫星标记：16个用于[植物名称1]，10个用于[植物名称2]，还有9个共同标记。[植物名称1]每个位点有1至12个等位基因，观察到的杂合度水平为0至0.923，预期杂合度水平为0.038至0.809。[植物名称2]每个位点有3至12个等位基因，观察到的杂合度水平为0至0.909，预期杂合度水平为0.127至0.797。有10个标记在[植物名称3]中可交叉扩增。
结论
杂交测序有助于经济高效地开发亲本物种的分子标记。这里开发的标记对于研究[植物名称]种群结构很有用。
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