Tian Yongqiang, Zhang Chunyun, Guo Mingquan
Sino-Africa Joint Research Center/Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
Graduate University of Chinese Academy of Sciences, Beijing 100049, China.
Molecules. 2015 Dec 7;20(12):21854-69. doi: 10.3390/molecules201219806.
The major active constituents from Amaryllidaceae family were reported to be Amaryllidaceae alkaloids (AAs), which exhibited a wide spectrum of biological activities, such as anti-tumor, anti-viral, and acetyl-cholinesterase-inhibitory activities. In order to better understand their potential as a source of bioactive AAs and the phytochemical variations among three different species of Lycoris herbs, the HPLC fingerprint profiles of Lycoris aurea (L. aurea), L. radiata, and L. guangxiensis were firstly determined and compared using LC-UV and LC-MS/MS. As a result, 39 peaks were resolved and identified as AAs, of which nine peaks were found in common for all these three species, while the other 30 peaks could be revealed as characteristic AAs for L. aurea, L. radiata and L. guangxiensis, respectively. Thus, these AAs can be used as chemical markers for the identification and quality control of these plant species. To further reveal correlations between chemical components and their pharmaceutical activities of these species at the molecular level, the bioactivities of the total AAs from the three plant species were also tested against HepG2 cells with the inhibitory rate at 78.02%, 84.91% and 66.81% for L. aurea, L. radiata and L. guangxiensis, respectively. This study firstly revealed that the three species under investigation were different not only in the types of AAs, but also in their contents, and both contributed to their pharmacological distinctions. To the best of our knowledge, the current research provides the most detailed phytochemical profiles of AAs in these species, and offers valuable information for future valuation and exploitation of these medicinal plants.
据报道，石蒜科植物的主要活性成分是石蒜科生物碱（AAs），其具有广泛的生物活性，如抗肿瘤、抗病毒和乙酰胆碱酯酶抑制活性。为了更好地了解它们作为生物活性AAs来源的潜力以及三种不同石蒜属草本植物之间的植物化学差异，首先使用液相色谱 - 紫外（LC - UV）和液相色谱 - 串联质谱（LC - MS/MS）测定并比较了忽地笑（L. aurea）、石蒜（L. radiata）和广西石蒜（L. guangxiensis）的高效液相色谱指纹图谱。结果，共分离并鉴定出39个峰为AAs，其中这三个物种共有9个峰，而其他30个峰分别可作为忽地笑、石蒜和广西石蒜的特征性AAs。因此，这些AAs可作为这些植物物种鉴定和质量控制的化学标记物。为了在分子水平上进一步揭示这些物种的化学成分与其药理活性之间的相关性，还测试了这三种植物物种总AAs对肝癌细胞HepG2的生物活性，忽地笑、石蒜和广西石蒜的抑制率分别为78.02%、84.91%和66.81%。本研究首次揭示，所研究的这三个物种不仅在AAs类型上不同，而且在其含量上也不同，两者共同导致了它们的药理差异。据我们所知，目前的研究提供了这些物种中AAs最详细的植物化学图谱，并为这些药用植物的未来评估和开发提供了有价值的信息。
