Alkhedaide Adel Qlayel
Medical Laboratory Department, University College - Turabah, Taif University, Taif, Saudi Arabia.
Antiinflamm Antiallergy Agents Med Chem. 2019;18(1):71-79. doi: 10.2174/1871523018666181126124336.
Chronic inflammation is a critical health issue and implicated in several chronic health problems such as tumors, auto-immune disorder, hypertension or diabetes. However, Juniperus procera is one of the famous ancient plants that has been traditionally used to treat several diseases such as hyperglycemia, hepatitis, jaundice, bronchitis, and pneumonia.
Current study is an attempt to investigate the anti-inflammatory effect of Juniperus procera extract on rats exposed to cytotoxicity caused experimentally by streptozotocin injections.
Five groups of adult Wistar rats (10 rats each) were examined as (Normal control, Normal rats treated with Juniperus procera extract, rats administrated with streptozotocin, rats administrated with streptozotocin and treated with insulin and, rats administrated with streptozotocin and Juniperus procera extract). At the end of the experiment, blood was collected from experimented rats. Animals then were killed and small parts of both pancreas and liver were collected for gene expression and histopathological examination.
Serum analysis showed a significant increase in glucose, IL-6, IL-2 and TNF-α levels in rats exposed to streptozotocin. That change was reduced in rats cotreated with insulin or Juniperus procera extract. Moreover, streptozotocin showed a significant upregulation of IL-6, TNF-α and A2M genes, while, either insulin or Juniperus procera treatment was restored to normal status. Streptozotocin induced inflammation within hepatic tissues which clearly reduced in hepatic tissues of both insulin and junipers cotreated groups.
Streptozotocin toxicity induces acute inflammation and increases serum glucose, IL-6, IL-2 and TNF-α levels. However, Juniperus procera extract was found to significantly prevent that reaction within four weeks experimented frame time.
慢性炎症是一个关键的健康问题，与多种慢性健康问题相关，如肿瘤、自身免疫性疾病、高血压或糖尿病。然而，刺柏是一种著名的古老植物，传统上被用于治疗多种疾病，如高血糖、肝炎、黄疸、支气管炎和肺炎。
本研究旨在探讨刺柏提取物对链脲佐菌素注射诱导的细胞毒性大鼠的抗炎作用。
将五组成年Wistar大鼠（每组10只）作为（正常对照组、用刺柏提取物处理的正常大鼠、注射链脲佐菌素的大鼠、注射链脲佐菌素并用胰岛素处理的大鼠、注射链脲佐菌素并用刺柏提取物处理的大鼠）进行检查。实验结束时，从实验大鼠采集血液。然后处死动物，收集胰腺和肝脏的小部分组织用于基因表达和组织病理学检查。
血清分析显示，暴露于链脲佐菌素的大鼠血糖、白细胞介素-6、白细胞介素-2和肿瘤坏死因子-α水平显著升高。在用胰岛素或刺柏提取物联合处理的大鼠中，这种变化有所减少。此外，链脲佐菌素显示白细胞介素-6、肿瘤坏死因子-α和α2-巨球蛋白基因显著上调，而胰岛素或刺柏处理均恢复到正常状态。链脲佐菌素诱导肝组织炎症，在胰岛素和刺柏联合处理组的肝组织中炎症明显减轻。
链脲佐菌素毒性诱导急性炎症并增加血清葡萄糖、白细胞介素-6、白细胞介素-2和肿瘤坏死因子-α水平。然而，在四周的实验时间内，发现刺柏提取物能显著预防这种反应。
