一种用于检测液体中脂褐素（细胞衰老的主要副产物）的新型定量方法。

A Novel Quantitative Method for the Detection of Lipofuscin, the Main By-Product of Cellular Senescence, in Fluids.

作者信息

Rizou Sophia V, Evangelou Konstantinos, Myrianthopoulos Vassilios, Mourouzis Iordanis, Havaki Sophia, Athanasiou Aikaterini, Vasileiou Panagiotis V S, Margetis Aggelos, Kotsinas Athanassios, Kastrinakis Nikolaos G, Sfikakis Petros, Townsend Paul, Mikros Emmanuel, Pantos Constantinos, Gorgoulis Vassilis G

机构信息

Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.

Department of Anatomy-Histology-Embryology, Medical School, University of Ioannina, Ioannina, Greece.

出版信息

Methods Mol Biol. 2019;1896:119-138. doi: 10.1007/978-1-4939-8931-7_12.

DOI:10.1007/978-1-4939-8931-7_12

PMID:30474845

Abstract

Lipofuscin accumulation is a hallmark of senescence. This nondegradable material aggregates in the cytoplasm of stressed or damaged cells due to metabolic imbalance associated with aging and age-related diseases. Indications of a soluble state of lipofuscin have also been provided, rendering the perspective of monitoring such processes via lipofuscin quantification in liquids intriguing. Therefore, the development of an accurate and reliable method is of paramount importance. Currently available assays are characterized by inherent pitfalls which demote their credibility. We herein describe a simple, highly specific and sensitive protocol for measuring lipofuscin levels in any type of liquid. The current method represents an evolution of a previously described assay, developed for in vitro and in vivo senescent cell recognition that exploits a newly synthesized Sudan Black-B analog (GL13). Analysis of human clinical samples with the modified protocol provided strong evidence of its usefulness for the exposure and surveillance of age-related conditions.

摘要

脂褐素积累是衰老的一个标志。这种不可降解的物质由于与衰老和年龄相关疾病相关的代谢失衡，在应激或受损细胞的细胞质中聚集。也有证据表明脂褐素处于可溶状态，这使得通过液体中脂褐素定量来监测此类过程的前景很有吸引力。因此，开发一种准确可靠的方法至关重要。目前可用的检测方法存在固有缺陷，这降低了它们的可信度。我们在此描述一种简单、高度特异且灵敏的方案，用于测量任何类型液体中的脂褐素水平。当前方法是先前描述的一种检测方法的改进，该检测方法是为体外和体内衰老细胞识别而开发的，利用了一种新合成的苏丹黑B类似物（GL13）。用改良方案对人类临床样本进行分析，有力地证明了其在暴露和监测与年龄相关状况方面的有用性。