School of Chemistry and Biological Engineering, Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation , Changsha University of Science and Technology , Changsha 410114 , People's Republic of China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering , Hunan University , Changsha 410082 , People's Republic of China.
Anal Chem. 2018 Dec 18;90(24):14514-14520. doi: 10.1021/acs.analchem.8b04355. Epub 2018 Dec 6.
Febrile seizure (FS), a frequently encountered seizure disorder in pediatric populations, can cause hippocampus damage. It has been elucidated that sulfur dioxide (SO) content is overproduced during the development of FS and related brain injury. Thus, monitoring in situ the level of endogenous SO in FS-related models is helpful to estimate the pathogenesis of FS-induced brain injury, but the effect detection method remains to be explored. Herein, we developed a two-photon energy transfer cassette based on an acedan-anthocyanidin scaffold, TP-Ratio-SO, allowing us to achieve this purpose. TP-Ratio-SO specifically responds to SO derivatives (HSO/SO) in an ultrafast fashion (less than 3 s), and HSO/SO can be sensitively determined with a detection limit of 26 nM. Moreover, it exhibits significant changes in two well-resolved fluorescence emissions (Δλ = 140 nm) by reacting with HSO/SO, behaving as a ratiometric fluorescent sensor. Importantly, ratiometric imaging of endogenous SO derivatives generation in hyperpyretic U251 cells and in a rat model of FS-treated hippocampus damage was successfully carried out by TP-Ratio-SO, demonstrating that it may be a promising tool for studying the role of SO in FS-associated neurological diseases.
热性惊厥（FS）是儿科人群中常见的癫痫发作疾病，可导致海马损伤。已经阐明，在 FS 及其相关脑损伤的发展过程中，二氧化硫（SO）含量过度产生。因此，在 FS 相关模型中原位监测内源性 SO 的水平有助于评估 FS 诱导的脑损伤的发病机制，但仍需探索其效应检测方法。在这里，我们基于acedan-anthocyanidin 支架开发了一种双光子能量转移盒，即 TP-Ratio-SO，使其能够实现这一目的。TP-Ratio-SO 可以快速（小于 3 秒）特异性响应 SO 衍生物（HSO/SO），并且可以通过检测限为 26 nM 的方法灵敏地确定 HSO/SO。此外，通过与 HSO/SO 反应，它在两个分辨率良好的荧光发射（Δλ=140nm）中表现出显著的变化，表现为比率荧光传感器。重要的是，通过 TP-Ratio-SO 成功地对高热 U251 细胞和 FS 处理的海马损伤大鼠模型中的内源性 SO 衍生物生成进行了比率成像，表明它可能是研究 SO 在 FS 相关神经疾病中的作用的有前途的工具。
