Chen Xiaoxiang, Xu Xueni, Pan Bei, Zeng Kaixuan, Xu Mu, Liu Xiangxiang, He Bangshun, Pan Yuqin, Sun Huiling, Wang Shukui
General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu, China.
Medical College, Southeast University, Nanjing 210009, Jiangsu, China.
Aging (Albany NY). 2018 Nov 26;10(11):3421-3437. doi: 10.18632/aging.101656.
MicroRNA-150-5p (miR-150-5p) has been implicated in tumor initiation and progression in a variety of cancers. However, its roles in colorectal cancer (CRC) remain largely unknown. In our study, a decreased miR-150-5p expression in CRC tissues was found to be associated with poor overall survival. Moreover, miR-150-5p inhibited CRC cell proliferation, migration, invasion and angiogenesis in vitro and in vivo, and its inhibitory effect could be reversed by transfection of vascular epithelial growth factor A (VEGFA) expression plasmid. Lastly, we demonstrated that miR-150-5p inactivated VEGFA/VEGFR2 and the downstream Akt/mTOR signaling pathway in CRC. Based on these results, we conclude that miR-150-5p may function as a tumor suppressor in CRC, and miR-150-5p/VEGFA axis may be a potential therapeutic target candidate in CRC treatment.
微小RNA-150-5p（miR-150-5p）已被证明与多种癌症的肿瘤发生和进展有关。然而，其在结直肠癌（CRC）中的作用仍 largely未知。在我们的研究中，发现CRC组织中miR-150-5p表达降低与总体生存率低相关。此外，miR-150-5p在体外和体内均抑制CRC细胞的增殖、迁移、侵袭和血管生成，并且通过转染血管内皮生长因子A（VEGFA）表达质粒可逆转其抑制作用。最后，我们证明miR-150-5p使CRC中的VEGFA/VEGFR2以及下游的Akt/mTOR信号通路失活。基于这些结果，我们得出结论，miR-150-5p可能在CRC中起肿瘤抑制作用，并且miR-150-5p/VEGFA轴可能是CRC治疗中潜在的治疗靶点候选物。
