Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Nat Methods. 2018 Dec;15(12):1011-1019. doi: 10.1038/s41592-018-0211-z. Epub 2018 Nov 26.
Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Since its introduction decades ago, the technique has grown to offer myriad implementations, each with its own strengths and weaknesses. We discuss these, aiming to provide a practical guide for biologists and to highlight which approach is best suited to a given application.
结构光照明显微镜（SIM）允许在活样本中进行快速、超分辨率（SR）成像。我们回顾了 SR-SIM 的最新技术进展，重点介绍了成像速度、分辨率和深度。自几十年前问世以来，该技术已经发展出了众多的实现方式，每种方式都有其自身的优缺点。我们讨论了这些方法，旨在为生物学家提供实用指南，并突出哪种方法最适合特定的应用。
