de Man A J, Foolen W J, van Dijk B A, Kunst V A, de Witte T M
Blood Transfusion Service, University Hospital, Nijmegen, The Netherlands.
Vox Sang. 1988;55(1):37-41. doi: 10.1111/j.1423-0410.1988.tb04686.x.
A method for the investigation of erythrocyte chimaerism in patients after bone marrow transplantation (BMT) has been developed using fluorescent microspheres coated with anti-human IgG. Blood group antigens different in patient and donor were used as marker. The specificity and reliability of this method was evaluated measuring artificial mixtures of blood group positive and negative red cells. Red cell antigens tested were D, c, K, Fya, Fyb, Jka, Jkb, S and s. Mean linear regression lines for mixtures with percentages positive cells ranging from 0.01 to 1% and 1 to 100% were 0.91X-0.03 (r = 0.99) and 1.00X-0.05 (r = 0.99), respectively. The sensitivity level of the assay was one positive cell per 10,000 blood group negative cells. In negative control samples (n = 15) a mean percentage positive cells was found of 0.002 +/- 0.004 (SD). Intra- and inter-assay coefficients of variation were 4.9 and 5.3% for mixtures of 10%, and were 11.1 and 24.6% for mixtures of 0.1% blood group positive cells. It is concluded that the described method can be used both to establish the take of donor marrow in an early phase after transplantation and to investigate mixed erythrocyte chimaerism long after BMT.
已开发出一种用于研究骨髓移植（BMT）后患者红细胞嵌合体的方法，该方法使用包被抗人IgG的荧光微球。患者和供体不同的血型抗原用作标记物。通过检测血型阳性和阴性红细胞的人工混合物来评估该方法的特异性和可靠性。检测的红细胞抗原包括D、c、K、Fya、Fyb、Jka、Jkb、S和s。阳性细胞百分比范围为0.01%至1%以及1%至100%的混合物的平均线性回归线分别为0.91X - 0.03（r = 0.99）和1.00X - 0.05（r = 0.99）。该检测方法的灵敏度水平为每10,000个血型阴性细胞中有一个阳性细胞。在阴性对照样本（n = 15）中，发现阳性细胞的平均百分比为0.002±0.004（标准差）。对于10%的混合物，批内和批间变异系数分别为4.9%和5.3%，对于0.1%血型阳性细胞的混合物，批内和批间变异系数分别为11.1%和24.6%。结论是，所描述的方法可用于在移植后的早期阶段确定供体骨髓是否植入，并用于研究骨髓移植后很长时间的混合红细胞嵌合体。
