1 Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si , Republic of Korea.
2 Yang Ju Livestock Industry Cooperatives , Gyeonggi, Republic of Korea.
Foodborne Pathog Dis. 2019 Apr;16(4):256-261. doi: 10.1089/fpd.2018.2509. Epub 2018 Nov 27.
In this study, we investigated the prevalence and characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates from pigs and pig farmers in Korea. A total of 1245 nasal swabs were collected from pigs and farmers at 53 pig farms in nine provinces from 2012 to 2016. The prevalence of MRSA was 7.9% (88/1119) in pigs and 16.7% (21/126) in farmers. Among 119 farmers working on conventional farms, 21 (17.6%) tested positive for MRSA. However, no MRSA was detected in either pigs or farmers at four organic farms. The individual pig-level prevalence of MRSA differed between age groups, with the percentage of positive samples being highest in weaners (17.3%), followed by growers (9.0%), piglets (6.5%), finishers (4.4%), and sows (0%). Two different lineages of MRSA were detected among the 109 isolates from pigs and farmers: (1) livestock-associated types of MRSA belonging to 27 isolates of ST541-t034-V, 24 isolates of ST398-t571-V, 17 isolates of ST398-t034 variant (G420A)-V, and one isolate of ST541-t8588-V; and (2) community-associated types of MRSA belonging to 21 isolates of ST188-t189-IV, 14 isolates of ST72-t324-IV, three isolates of ST72-t664-IV, one isolate of ST72-t148-IV, and one isolate of ST72-t5440-IV. Identical genotypes were detected in both pigs and farmers at six farms. New MRSA clones were isolated from pigs (ST398-t034 (G420A)-V, ST188-t189-IV, and ST398-t571-V) and pig farmers (ST541-t8588-V and ST72-t5440-IV). The prevalence of MRSA was also correlated with antibiotic usage, and the clones showed diversification between pigs and farmers. Ultimately, the increased frequency and diversification of livestock-associated MRSA could be a potential threat to public health in Korea. To our knowledge, this is the first study to report the prevalence and characteristics of MRSA in pigs and pig farmers in Korea.
在这项研究中，我们调查了韩国猪和猪农中耐甲氧西林金黄色葡萄球菌（MRSA）分离株的流行情况和特征。我们从 2012 年至 2016 年从 9 个省的 53 个养猪场收集了 1245 份鼻拭子，来自猪和农民。MRSA 在猪中的流行率为 7.9%（88/1119），在农民中为 16.7%（21/126）。在 119 名从事常规农场工作的农民中，有 21 名（17.6%）对 MRSA 检测呈阳性。然而，在四个有机农场，无论是猪还是农民，均未检测到 MRSA。个体猪水平的 MRSA 流行率因年龄组而异，其中断奶仔猪（17.3%）的阳性样本比例最高，其次是育肥猪（9.0%）、仔猪（6.5%）、肥育猪（4.4%）和母猪（0%）。从猪和农民的 109 株分离物中检测到两种不同的 MRSA 谱系：（1）属于 27 株 ST541-t034-V、24 株 ST398-t571-V、17 株 ST398-t034 变体（G420A）-V 和 1 株 ST541-t8588-V 的畜群相关型 MRSA；（2）属于 21 株 ST188-t189-IV、14 株 ST72-t324-IV、3 株 ST72-t664-IV、1 株 ST72-t148-IV 和 1 株 ST72-t5440-IV 的社区相关型 MRSA。在六个农场中，均在猪和农民中检测到相同的基因型。从猪（ST398-t034（G420A）-V、ST188-t189-IV 和 ST398-t571-V）和猪农民（ST541-t8588-V 和 ST72-t5440-IV）中分离出新的 MRSA 克隆。MRSA 的流行率与抗生素的使用有关，并且克隆在猪和农民之间表现出多样化。最终，畜群相关的 MRSA 的频率增加和多样化可能对韩国的公共卫生构成潜在威胁。据我们所知，这是第一项在韩国报告猪和猪农中 MRSA 流行情况和特征的研究。
