Parks Robyn N, Kim Christopher J, Al-Safi Zain A, Armstrong Abigail A, Zore Temeka, Moatamed Neda A
1 University of California, Los Angeles, CA, USA.
2 Marin Medical Laboratories, Greenbrae, CA, USA.
Int J Surg Pathol. 2019 Jun;27(4):372-379. doi: 10.1177/1066896918814307. Epub 2018 Nov 28.
Chronic endometritis is characterized by plasma cell (PC) infiltration of endometrial stroma. Identification of PCs can be challenging by routine hematoxylin and eosin (H&E) stain due to the low numbers of PCs or to their being obscured by other cells in the stroma. CD138 is widely used as an ancillary immunohistochemistry stain to identify PCs; however, it has a high background reaction. In this study, multiple myeloma 1 (MUM1) transcription factor is introduced as an alternative PC marker in endometrial tissues. In this study, 311 endometrial biopsies, submitted to rule out chronic endometritis, were selected. They were divided into Group I (n = 87) and Group II (n = 224). Both had MUM1 and H&E while Group I also had accompanying CD138 stains. In both groups combined, MUM1 detected plasma cells in 48% of the cases, while CD138 and H&E identified the cells in 23% and 15% of the biopsies, respectively. In addition to having a clean background, MUM1 is a more sensitive stain than CD138 for detection of PCs in endometrium.
慢性子宫内膜炎的特征是子宫内膜间质中有浆细胞浸润。由于浆细胞数量较少或被间质中的其他细胞掩盖，通过常规苏木精和伊红（H&E）染色来识别浆细胞具有挑战性。CD138被广泛用作辅助免疫组织化学染色来识别浆细胞；然而，它有较高的背景反应。在本研究中，引入多发性骨髓瘤1（MUM1）转录因子作为子宫内膜组织中浆细胞的替代标记物。在本研究中，选取了311例为排除慢性子宫内膜炎而进行的子宫内膜活检样本。它们被分为I组（n = 87）和II组（n = 224）。两组均进行了MUM1和H&E染色，而I组还同时进行了CD138染色。在两组样本合并统计时，MUM1在48%的病例中检测到了浆细胞，而CD138和H&E分别在23%和15%的活检样本中识别出了浆细胞。除了背景清晰外，MUM1在检测子宫内膜中的浆细胞方面比CD138更敏感。
