Department of Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, California, USA.
Departments of Human Biology and Genomic Medicine, J. Craig Venter Institute, Rockville, Maryland, USA.
mBio. 2018 Nov 27;9(6):e01631-18. doi: 10.1128/mBio.01631-18.
To address the question of how microbial diversity and function in the oral cavities of children relates to caries diagnosis, we surveyed the supragingival plaque biofilm microbiome in 44 juvenile twin pairs. Using shotgun sequencing, we constructed a genome encyclopedia describing the core supragingival plaque microbiome. Caries phenotypes contained statistically significant enrichments in specific genome abundances and distinct community composition profiles, including strain-level changes. Metabolic pathways that are statistically associated with caries include several sugar-associated phosphotransferase systems, antimicrobial resistance, and metal transport. Numerous closely related previously uncharacterized microbes had substantial variation in central metabolism, including the loss of biosynthetic pathways resulting in auxotrophy, changing the ecological role. We also describe the first complete genomes from the human microbiome. Caries is a microbial community metabolic disorder that cannot be described by a single etiology, and our results provide the information needed for next-generation diagnostic tools and therapeutics for caries. Oral health has substantial economic importance, with over $100 billion spent on dental care in the United States annually. The microbiome plays a critical role in oral health, yet remains poorly classified. To address the question of how microbial diversity and function in the oral cavities of children relate to caries diagnosis, we surveyed the supragingival plaque biofilm microbiome in 44 juvenile twin pairs. Using shotgun sequencing, we constructed a genome encyclopedia describing the core supragingival plaque microbiome. This unveiled several new previously uncharacterized but ubiquitous microbial lineages in the oral microbiome. Caries is a microbial community metabolic disorder that cannot be described by a single etiology, and our results provide the information needed for next-generation diagnostic tools and therapeutics for caries.
为了解儿童口腔微生物多样性和功能与龋齿诊断之间的关系，我们调查了 44 对青少年双胞胎的龈上菌斑生物膜微生物组。通过 shotgun 测序，我们构建了一个基因组百科全书，描述了核心龈上菌斑微生物组。龋齿表型在特定的基因组丰度和独特的群落组成谱中包含有统计学意义的富集，包括菌株水平的变化。与龋齿相关的代谢途径包括几个与糖相关的磷酸转移酶系统、抗菌药物耐药性和金属运输。许多密切相关的以前未被描述的微生物在中心代谢中有很大的变化，包括导致营养缺陷的生物合成途径的丧失，从而改变了生态角色。我们还描述了人类微生物组的第一个完整基因组。龋齿是一种微生物群落代谢紊乱，不能用单一病因来描述，我们的结果为下一代龋齿诊断工具和治疗方法提供了所需的信息。口腔健康具有重要的经济意义，美国每年在牙科保健上的花费超过 1000 亿美元。微生物组在口腔健康中起着至关重要的作用，但仍未得到很好的分类。为了解儿童口腔微生物多样性和功能与龋齿诊断之间的关系，我们调查了 44 对青少年双胞胎的龈上菌斑生物膜微生物组。通过 shotgun 测序，我们构建了一个基因组百科全书，描述了核心龈上菌斑微生物组。这揭示了口腔微生物组中几个以前未被描述但普遍存在的新微生物谱系。龋齿是一种微生物群落代谢紊乱，不能用单一病因来描述，我们的结果为下一代龋齿诊断工具和治疗方法提供了所需的信息。
