Department of Conservative Dentistry and Periodontics, Affiliated Hospital of Stomatology, College of Medicine, Zhejiang University, Hangzhou, China,
Curr Microbiol. 2013 Nov;67(5):537-42. doi: 10.1007/s00284-013-0393-7. Epub 2013 Jun 7.
Severe early childhood caries are a prevalent public health problem among preschool children throughout the world. However, little is known about the microbiota found in association with severe early childhood caries. Our study aimed to explore the bacterial microbiota of dental plaques to study the etiology of severe early childhood caries through pyrosequencing analysis based on 16S rRNA gene V1-V3 hypervariable regions. Forty participants were enrolled in the study, and we obtained twenty samples of supragingival plaque from caries-free subjects and twenty samples from subjects with severe early childhood caries. A total of 175,918 reads met the quality control standards, and the bacteria found belonged to fourteen phyla and sixty-three genera. Our results show the overall structure and microbial composition of oral bacterial communities, and they suggest that these bacteria may present a core microbiome in the dental plaque microbiota. Three genera, Streptococcus, Granulicatella, and Actinomyces, were increased significantly in children with severe dental cavities. These data may facilitate improvements in the prevention and treatment of severe early childhood caries.
严重婴幼儿龋齿是全世界学龄前儿童中普遍存在的公共卫生问题。然而，对于与严重婴幼儿龋齿相关的微生物群了解甚少。我们的研究旨在通过基于 16S rRNA 基因 V1-V3 高变区的焦磷酸测序分析，探索牙菌斑中的细菌微生物群，以研究严重婴幼儿龋齿的病因。共有 40 名参与者入组研究，我们从无龋儿童中获得了 20 个龈上菌斑样本，从严重婴幼儿龋齿儿童中获得了 20 个样本。共有 175918 个符合质量控制标准的读取物，所发现的细菌属于十四个门和六十三属。我们的结果显示了口腔细菌群落的整体结构和微生物组成，并表明这些细菌可能在牙菌斑微生物群中呈现核心微生物组。三个属，链球菌、颗粒链菌和放线菌，在患有严重龋齿的儿童中显著增加。这些数据可能有助于改善严重婴幼儿龋齿的预防和治疗。
