Detection of methicillin-resistant (MRSA) in clinical samples of patients with external ocular infection.

BACKGROUND AND OBJECTIVES

is the main Gram-positive bacteria isolated from patients with ocular infections. Herein, we describe the pattern of antibiotic resistance, presence of resistance genes including and the cytotoxin gene in isolates collected from patients with external ocular infection.

MATERIALS AND METHODS

In this study, 8 isolates were collected from 81 patients that suffered from eye damage. Antibacterial susceptibility of isolates was determined using the Kirby-Bauer disk diffusion method. Resistance genes including and the virulence gene were detected by PCR method. Staphylococcal cassette chromosome (SCC) in MRSA isolates were detected by the multiplex-PCR method.

RESULTS

Three isolates were resistant to cefoxitin which is considered MRSA. The gene was identified in MRSA isolates. SCC type IV and the gene were detected in one of the MRSA isolates that was recovered from a diabetic patient.

CONCLUSION

The emergence of isolates belonging to SCC type IV and gene among patients with ocular infection is very serious; therefore, identify genetic characterization of MRSA isolates for empirical therapy and infection control is very important.