Faridi Ashkan, Kareshk Amir Tavakoli, Fatahi-Bafghi Mehdi, Ziasistani Mahsa, Ghahraman Mohammad Reza Kandehkar, Seyyed-Yousefi Seyedeh Zeinab, Shakeri Noshin, Kalantar-Neyestanaki Davood
Student Research Committee, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Iran J Microbiol. 2018 Aug;10(4):215-219.
is the main Gram-positive bacteria isolated from patients with ocular infections. Herein, we describe the pattern of antibiotic resistance, presence of resistance genes including and the cytotoxin gene in isolates collected from patients with external ocular infection.
In this study, 8 isolates were collected from 81 patients that suffered from eye damage. Antibacterial susceptibility of isolates was determined using the Kirby-Bauer disk diffusion method. Resistance genes including and the virulence gene were detected by PCR method. Staphylococcal cassette chromosome (SCC) in MRSA isolates were detected by the multiplex-PCR method.
Three isolates were resistant to cefoxitin which is considered MRSA. The gene was identified in MRSA isolates. SCC type IV and the gene were detected in one of the MRSA isolates that was recovered from a diabetic patient.
The emergence of isolates belonging to SCC type IV and gene among patients with ocular infection is very serious; therefore, identify genetic characterization of MRSA isolates for empirical therapy and infection control is very important.
是从眼部感染患者中分离出的主要革兰氏阳性菌。在此，我们描述了从眼外感染患者中收集的分离株的抗生素耐药模式、包括和细胞毒素基因在内的耐药基因的存在情况。
在本研究中，从81例眼部受损患者中收集了8株分离株。采用 Kirby-Bauer 纸片扩散法测定分离株的抗菌药敏性。通过PCR方法检测包括和毒力基因在内的耐药基因。采用多重PCR方法检测耐甲氧西林金黄色葡萄球菌（MRSA）分离株中的葡萄球菌盒式染色体（SCC）。
3株分离株对头孢西丁耐药，被认为是MRSA。在MRSA分离株中鉴定出基因。在1株从糖尿病患者中分离出的MRSA分离株中检测到IV型SCC和基因。
眼部感染患者中出现属于IV型SCC和基因的分离株非常严重；因此，鉴定MRSA分离株的基因特征对于经验性治疗和感染控制非常重要。
