Cancer Cell Biology Programme, Growth factors, Nutrients and Cancer Group, Centro Nacional Investigaciones Oncológicas, CNIO, Madrid, Spain.
Adv Exp Med Biol. 2018;1106:95-108. doi: 10.1007/978-3-030-00737-9_7.
Almost 15 years ago, the URI prefoldin-like complex was discovered by Krek and colleagues in immunoprecipitation experiments conducted in mammalian cells with the aim of identifying new binding partners of the E3 ubiquitin-protein ligase S-phase kinase-associated protein 2 (SKP2) (Gstaiger et al. Science 302(5648):1208-1212, 2003). The URI prefoldin-like complex is a heterohexameric chaperone complex comprising two α and four β subunits (α2β4). The α subunits are URI and STAP1, while the β subunits are PFDN2, PFDN6, and PFDN4r, one of which is probably present in duplicate. Elucidating the roles and functions of these components in vitro and in vivo will help to clarify the mechanistic behavior of what appears to be a remarkably important cellular machine.
大约 15 年前，Krek 及其同事在哺乳动物细胞中进行的免疫沉淀实验中发现了 URI 原核前体样复合物，目的是鉴定 E3 泛素蛋白连接酶 S 期激酶相关蛋白 2 (SKP2) 的新结合伴侣 (Gstaiger 等人，《科学》302(5648):1208-1212, 2003)。URI 原核前体样复合物是一种异六聚体伴侣复合物，由两个 α 和四个 β 亚基（α2β4）组成。α 亚基是 URI 和 STAP1，而 β 亚基是 PFDN2、PFDN6 和 PFDN4r，其中一个可能是重复的。阐明这些成分在体外和体内的作用和功能将有助于阐明这一似乎非常重要的细胞机器的机械行为。
