Prezioso Carla, Moens Ugo, Oliveto Giuseppe, Brazzini Gabriele, Piacentini Francesca, Frasca Federica, Viscido Agnese, Scordio Mirko, Guerrizio Giuliana, Rodio Donatella Maria, Pierangeli Alessandra, d'Ettorre Gabriella, Turriziani Ombretta, Antonelli Guido, Scagnolari Carolina, Pietropaolo Valeria
Department of Public Health and Infectious Diseases, "Sapienza" University of Rome, 00185 Rome, Italy.
IRCSS San Raffaele Pisana, Microbiology of Chronic Neuro-Degenerative Pathologies, 00163 Rome, Italy.
Microorganisms. 2021 Jun 9;9(6):1259. doi: 10.3390/microorganisms9061259.
Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been declared a global pandemic. Our goal was to determine whether co-infections with respiratory polyomaviruses, such as Karolinska Institutet polyomavirus (KIPyV) and Washington University polyomavirus (WUPyV) occur in SARS-CoV-2 infected patients. Oropharyngeal swabs from 150 individuals, 112 symptomatic COVID-19 patients and 38 healthcare workers not infected by SARS-CoV-2, were collected from March 2020 through May 2020 and tested for KIPyV and WUPyV DNA presence. Of the 112 SARS-CoV-2 positive patients, 27 (24.1%) were co-infected with KIPyV, 5 (4.5%) were positive for WUPyV, and 3 (2.7%) were infected simultaneously by KIPyV and WUPyV. Neither KIPyV nor WUPyV DNA was detected in samples of healthcare workers. Significant correlations were found in patients co-infected with SARS-CoV-2 and KIPyV ( < 0.05) and between SARS-CoV-2 cycle threshold values and KIPyV, WUPyV and KIPyV and WUPyV concurrently detected ( < 0.05). These results suggest that KIPyV and WUPyV may behave as opportunistic respiratory pathogens. Additional investigations are needed to understand the epidemiology and the prevalence of respiratory polyomavirus in COVID-19 patients and whether KIPyV and WUPyV could potentially drive viral interference or influence disease outcomes by upregulating SARS-CoV-2 replicative potential.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）已被宣布为全球大流行病。我们的目标是确定感染SARS-CoV-2的患者是否会同时感染呼吸道多瘤病毒，如卡罗林斯卡学院多瘤病毒（KIPyV）和华盛顿大学多瘤病毒（WUPyV）。2020年3月至2020年5月期间，收集了150人的口咽拭子，其中包括112例有症状的COVID-19患者和38名未感染SARS-CoV-2的医护人员，并检测了KIPyV和WUPyV DNA的存在情况。在112例SARS-CoV-2阳性患者中，27例（24.1%）同时感染了KIPyV，5例（4.5%）WUPyV呈阳性，3例（2.7%）同时感染了KIPyV和WUPyV。医护人员的样本中未检测到KIPyV和WUPyV DNA。在同时感染SARS-CoV-2和KIPyV的患者中发现了显著相关性（<0.05），以及在SARS-CoV-2循环阈值与KIPyV、WUPyV以及同时检测到的KIPyV和WUPyV之间也发现了显著相关性（<0.05）。这些结果表明，KIPyV和WUPyV可能作为机会性呼吸道病原体。需要进一步调查以了解COVID-19患者中呼吸道多瘤病毒的流行病学和患病率，以及KIPyV和WUPyV是否可能通过上调SARS-CoV-2的复制潜力来驱动病毒干扰或影响疾病结局。
