Department of Public Health and Infectious Diseases, "Sapienza" University, P.le Aldo Moro, 5, 00185, Rome, Italy.
Laboratory of Clinical Microbiology and Virology, Polyclinic Tor Vergata Foundation, Rome, Italy.
Med Microbiol Immunol. 2019 Dec;208(6):845-854. doi: 10.1007/s00430-019-00630-9. Epub 2019 Aug 2.
Fragmented data are available on the human polyomaviruses (HPyVs) prevalence in the gastrointestinal tract. Rearrangements in the non-coding control region (NCCR) of JCPyV and BKPyV have been extensively studied and correlated to clinical outcome; instead, little information is available for KIPyV, WUPyV and MCPyV NCCRs. To get insights into the role of HPyVs in the gastrointestinal tract, we investigated JCPyV, BKPyV, KIPyV, WUPyV and MCPyV distribution among hematological patients in concomitance with gastrointestinal symptoms. In addition, NCCRs and VP1 sequences were examined to characterize the strains circulating among the enrolled patients. DNA was extracted from 62 stool samples and qPCR was carried out to detect and quantify JCPyV, BKPyV, KIPyV, WUPyV and MCPyV genomes. Positive samples were subsequently amplified and sequenced for NCCR and VP1 regions. A phylogenetic tree was constructed aligning the obtained VP1 sequences to a set of reference sequences. qPCR revealed low viral loads for all HPyVs searched. Mono and co-infections were detected. A significant correlation was found between gastrointestinal complications and KIPyV infection. Archetype-like NCCRs were found for JCPyV and BKPyV, and a high degree of NCCRs stability was observed for KIPyV, WUPyV and MCPyV. Analysis of the VP1 sequences revealed a 99% identity with the VP1 reference sequences. The study adds important information on HPyVs prevalence and persistence in the gastrointestinal tract. Gastrointestinal signs were correlated with the presence of KIPyV, although definitive conclusions cannot be drawn. HPyVs NCCRs showed a high degree of sequence stability, suggesting that sequence rearrangements are rare in this anatomical site.
关于人类多瘤病毒（HPyV）在胃肠道中的流行情况，已有零散的数据可供参考。JCPyV 和 BKPyV 的非编码控制区（NCCR）的重排已得到广泛研究，并与临床结果相关；相比之下，关于 KIPyV、WUPyV 和 MCPyV NCCR 的信息较少。为了深入了解 HPyV 在胃肠道中的作用，我们研究了血液系统疾病患者同时伴有胃肠道症状时 JCPyV、BKPyV、KIPyV、WUPyV 和 MCPyV 的分布情况。此外，还对 NCCR 和 VP1 序列进行了检测，以分析在入组患者中循环的病毒株。从 62 份粪便样本中提取 DNA，通过 qPCR 检测和定量 JCPyV、BKPyV、KIPyV、WUPyV 和 MCPyV 基因组。随后对阳性样本进行 NCCR 和 VP1 区域的扩增和测序。将获得的 VP1 序列与一组参考序列进行比对构建系统发育树。qPCR 显示所有搜索到的 HPyV 病毒载量均较低。检测到单重和多重感染。发现胃肠道并发症与 KIPyV 感染显著相关。JCPyV 和 BKPyV 存在典型的 NCCR，而 KIPyV、WUPyV 和 MCPyV 的 NCCR 稳定性较高。VP1 序列分析显示与 VP1 参考序列的同一性为 99%。该研究提供了有关 HPyV 在胃肠道中流行和持续存在的重要信息。胃肠道症状与 KIPyV 的存在相关，但不能得出明确的结论。HPyV 的 NCCR 显示出高度的序列稳定性，表明在该解剖部位序列重排很少发生。
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