Yang Xiao, Jeong Woonhyeok, Son Daegu, Ryoo Youngwook, Kim Jinhee, Oh Youngkee, Kwon Sunyoung, Liu Dalie
Department of Plastic and Reconstructive Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Department of Plastic and Reconstructive Surgery, Institute for Medical Science, Dongsan Medical Center, Keimyung University, Daegu, Republic of Korea.
Wounds. 2019 Feb;31(2):59-64. Epub 2018 Nov 22.
Radiation-delayed wounds require diverse therapeutic strategies to achieve effective healing. However, the development of novel therapies with a radiation-delayed wound healing model is hindered by the lack of standardized animal models.
In this study, the authors propose and verify a procedure to establish a radiation-delayed wound healing model in pigs.
Two female pigs received a single 18-Gy dose of a 6-MeV electron beam per 18 cm x 8 cm area. Three areas were treated on the paraspinal dorsal skin surface of each pig, with 2 on the left side of the spine and 1 on the right. Wounds were periodically created on the 2 pigs at 1 of the following time points: (1) 2 weeks post radiation (PR2 group; n = 4), (2) 4 weeks post radiation (PR4 group; n = 4), and (3) 6 weeks post radiation (PR6 group; n = 4). A partial-thickness wound was created by excising the skin, superficial fat layer, and superficial fascia while preserving the deep fat and deep fascia. Wound contraction was evaluated, and histological analysis was performed at 2 and 4 weeks after wounding.
The control wounds displayed complete reepithelialization at week 4. However, the PR6 group showed delayed wound healing for the entire experimental period. Furthermore, compared with the control group, the PR6 group demonstrated excessive acute and chronic inflammation and exhibited incomplete reepithelialization at week 4.
These findings suggest skin wounding 6 weeks after irradiation is most suitable for the induction of a delayed wound healing model. Using this protocol, the authors safely generated a delayed wound healing model without acute complications from irradiation.
放射性延迟伤口需要多种治疗策略才能实现有效愈合。然而，缺乏标准化的动物模型阻碍了利用放射性延迟伤口愈合模型开发新疗法。
在本研究中，作者提出并验证了一种在猪身上建立放射性延迟伤口愈合模型的方法。
两只雌性猪每18 cm×8 cm区域接受单次18 Gy剂量的6 MeV电子束照射。在每只猪的脊柱旁背部皮肤表面处理三个区域，脊柱左侧两个，右侧一个。在以下时间点之一对两只猪定期造创：（1）放疗后2周（PR2组；n = 4），（2）放疗后4周（PR4组；n = 4），以及（3）放疗后6周（PR6组；n = 4）。通过切除皮肤、浅脂肪层和浅筋膜，同时保留深脂肪和深筋膜来创建部分厚度伤口。评估伤口收缩情况，并在造创后2周和4周进行组织学分析。
对照伤口在第4周显示完全再上皮化。然而，PR6组在整个实验期间伤口愈合延迟。此外，与对照组相比，PR6组表现出过度的急性和慢性炎症，并且在第4周时再上皮化不完全。
这些发现表明放疗后6周进行皮肤造创最适合诱导延迟伤口愈合模型。使用该方案，作者安全地建立了一个延迟伤口愈合模型，且无放疗引起的急性并发症。
