Light, Nanomaterials and Nanotechnology (L2N), FRE-CNRS 2019, Institute Charles Delaunay (ICD), University of Technology of Troyes, 12 Rue Marie Curie CS 42060, 10004 Troyes CEDEX, France.
Laboratory Interdisciplinaire Carnot de Bourgogne (ICB), UMR-CNRS 6303, University of Bourgogne Franche-Comté, 9 Avenue Alain Savary, 21078 Dijon CEDEX, France.
Biosensors (Basel). 2018 Nov 27;8(4):118. doi: 10.3390/bios8040118.
Conductive indium-tin oxide (ITO) and non-conductive glass substrates were successfully modified with embedded gold nanoparticles (AuNPs) formed by controlled thermal annealing at 550 °C for 8 h in a preselected oven. The authors characterized the formation of AuNPs using two microscopic techniques: scanning electron microscopy (SEM) and atomic force microscopy (AFM). The analytical performances of the nanostructured-glasses were compared regarding biosensing of Hsp70, an ATP-driven molecular chaperone. In this work, the human heat-shock protein (Hsp70), was chosen as a model biomarker of body stress disorders for microwave spectroscopic investigations. It was found that microwave screening at 4 GHz allowed for the first time the detection of 12 ng/µL/cm² of Hsp70.
经 550°C 下 8 小时的预设定烤箱热退火处理，成功在导电铟锡氧化物（ITO）和非导电玻璃衬底上嵌入金纳米颗粒（AuNPs）。作者采用扫描电子显微镜（SEM）和原子力显微镜（AFM）两种微观技术来对 AuNPs 的形成进行了表征。对纳米结构玻璃的分析性能进行了比较，研究了其对热休克蛋白 70（Hsp70）的生物传感性能，Hsp70 是一种 ATP 驱动的分子伴侣，是生物体内应激紊乱的生物标志物。在这项工作中，人类热休克蛋白（Hsp70）被选为微波光谱研究的生物标志物模型。研究发现，在 4GHz 微波筛选下，首次检测到了 12ng/µL/cm²的 Hsp70。
