Saelao Perot, Wang Ying, Chanthavixay Ganrea, Yu Vivian, Gallardo Rodrigo A, Dekkers Jack C M, Lamont Susan J, Kelly Terra, Zhou Huaijun
Integrative Genetics and Genomics Graduate Group, University of California, Davis, CA 95616, USA.
Genomics to Improve Poultry Innovation Lab, University of California, Davis, CA 95616, USA.
Genes (Basel). 2018 Nov 27;9(12):579. doi: 10.3390/genes9120579.
Newcastle disease virus (NDV) is a devastating worldwide poultry pathogen with major implications for global food security. In this study, two highly inbred and genetically distinct chicken lines, Fayoumis and Leghorns, were exposed to a lentogenic strain of NDV, while under the effects of heat stress, in order to understand the genetic mechanisms of resistance during high ambient temperatures. Fayoumis, which are relatively more resistant to pathogens than Leghorns, had larger numbers of differentially expressed genes (DEGs) during the early stages of infection when compared to Leghorns and subsequently down-regulated their immune response at the latter stages to return to homeostasis. Leghorns had very few DEGs across all observed time points, with the majority of DEGs involved with metabolic and glucose-related functions. Proteomic analysis corroborates findings made within Leghorns, while also identifying interesting candidate genes missed by expression profiling. Poor correlation between changes observed in the proteomic and transcriptomic datasets highlights the potential importance of integrative approaches to understand the mechanisms of disease response. Overall, this study provides novel insights into global protein and expression profiles of these two genetic lines, and provides potential genetic targets involved with NDV resistance during heat stress in poultry.
新城疫病毒（NDV）是一种在全球范围内极具破坏力的家禽病原体，对全球粮食安全有着重大影响。在本研究中，为了解高温环境下抗性的遗传机制，将两个高度近交且基因不同的鸡品系，即法尤米鸡和来航鸡，暴露于一种缓发型新城疫病毒毒株，同时使其处于热应激状态。与来航鸡相比，法尤米鸡对病原体的抗性相对更强，在感染早期，其差异表达基因（DEG）的数量比来航鸡更多，随后在后期下调其免疫反应以恢复体内平衡。在所有观察时间点，来航鸡的DEG数量很少，大多数DEG与代谢和葡萄糖相关功能有关。蛋白质组学分析证实了来航鸡的研究结果，同时还鉴定出了表达谱分析遗漏的有趣候选基因。蛋白质组学和转录组学数据集中观察到的变化之间的相关性较差，这凸显了综合方法在理解疾病反应机制方面的潜在重要性。总体而言，本研究为这两个基因品系的整体蛋白质和表达谱提供了新见解，并提供了家禽在热应激期间与新城疫病毒抗性相关的潜在遗传靶点。
