College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Department of Animal Production, Faculty of Agriculture, Sana'a University, Alwehdah Street, Sana'a P.O. Box 19509, Yemen.
Genes (Basel). 2022 Dec 28;14(1):95. doi: 10.3390/genes14010095.
Meat quality traits are an important economic trait and remain a major argument, from the producer to the consumer. However, there are a few candidate genes and pathways of chicken meat quality traits that were reported for chicken molecular breeding. The purpose of the present study is to identify the candidate genes and pathways associated with meat quality underlying variations in meat quality. Hence, transcriptome profiles of breast tissue in commercial Digao (DG, 5 male) and Chahua (CH, 5 male) native chicken breeds were analyzed at the age of 100 days. The results found 3525 differentially expressed genes (DEGs) in CH compared to DG with adjusted -values of ≤0.05 and log2FC ≥ 0.1 FDR ≤ 0.05. Functional analysis of GO showed that the DEGs are mainly involved in the two types of processes of meat quality, such as positive regulation of the metabolic process, extracellular structure organization, collagen trimer, cellular amino acid metabolic process, cellular amino acid catabolic process, and heme binding. Functional analysis of KEGG showed that the DEGs are mainly involved in the two types of processes of meat quality, such as oxidative phosphorylation, carbon metabolism, valine, leucine, and isoleucine degradation, and fatty acid degradation. Many of the DEGs are well known to be related to meat quality, such as and . Therefore, the current study detected multiple pathways and genes that could be involved in the control of the meat quality traits of chickens. These findings should be used as an essential resource to improve the accuracy of selection for meat traits in chickens using marker-assisted selection based on differentially expressed genes.
肉质特性是一个重要的经济性状，一直是生产者和消费者争论的主要话题。然而，在鸡分子育种方面，仅有少数候选基因和途径被报道与鸡肉质性状有关。本研究旨在鉴定与肉质差异相关的候选基因和途径。因此，在 100 日龄时分析了商用狄高（DG，5 只雄性）和茶花（CH，5 只雄性）本地鸡种的胸组织转录组图谱。结果发现，CH 与 DG 相比有 3525 个差异表达基因（DEGs），调整后的 P 值≤0.05，log2FC≥0.1， FDR≤0.05。GO 功能分析表明，DEGs 主要参与肉质的两种类型的过程，如代谢过程的正调控、细胞外结构组织、胶原蛋白三聚体、细胞氨基酸代谢过程、细胞氨基酸分解代谢过程和血红素结合。KEGG 功能分析表明，DEGs 主要参与肉质的两种类型的过程，如氧化磷酸化、碳代谢、缬氨酸、亮氨酸和异亮氨酸降解以及脂肪酸降解。许多 DEGs 被认为与肉质有关，如 和 。因此，本研究检测到了多个可能参与控制鸡肉质性状的途径和基因。这些发现应该被用作一个基本资源，以提高基于差异表达基因的标记辅助选择对鸡肉质性状选择的准确性。
