Morales Agustin Martin, Mukai Rie, Murota Kaeko, Terao Junji
Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.
Department of Life Sciecne, Faculty of Science and Engeering, Kindai University, 3-4-1 Kowakae, Higashi-Osaka, Osaka 577-8502, Japan.
J Clin Biochem Nutr. 2018 Nov;63(3):175-180. doi: 10.3164/jcbn.18-38. Epub 2018 Jul 12.
To determine the preventive effect of dietary rutin on oxidative damages occurring in the digestive tract, 13-hydroperoxyoctadecadienoic acid and hemoglobin were exposed to Caco-2 intestinal cells after the pretreatment with colonic rutin metabolites. Among four catechol-type metabolites, quercetin and 3,4-dihydroxytoluene exerted significant protection on 13-hydroperoxyoctadecadienoic and hemoglobin-dependent lipid peroxidation of this epithelial cell. Compared with quercetin, a much lower concentration allowed 3,4-dihydroxytoluene to maximize the protective effect, though it needed a longer pre-incubation period. Neither quercetin nor 3,4-dihydroxytoluene affected the expression of peroxiredoxin-6 protein, which comprises the cellular antioxidant defense system. It is concluded that 3,4-dihydroxytoluene is a plausible rutin colonic metabolite that can suppress oxidative damages of intestinal epithelial cells by directly inhibiting lipid peroxidation. This result may illuminate the preventive role of dietary rutin against colorectal cancer incidence in relation to the consumption of red and processed meat.
为确定膳食芦丁对消化道发生的氧化损伤的预防作用，在用结肠芦丁代谢物预处理后，将13-氢过氧化十八碳二烯酸和血红蛋白暴露于Caco-2肠细胞。在四种儿茶酚型代谢物中，槲皮素和3,4-二羟基甲苯对该上皮细胞的13-氢过氧化十八碳二烯酸和血红蛋白依赖性脂质过氧化具有显著的保护作用。与槲皮素相比，虽然3,4-二羟基甲苯需要更长的预孵育时间，但更低的浓度就能使其保护作用最大化。槲皮素和3,4-二羟基甲苯均未影响过氧化物还原酶-6蛋白的表达，而过氧化物还原酶-6蛋白构成细胞抗氧化防御系统。得出结论，3,4-二羟基甲苯是一种可能的芦丁结肠代谢物，它可通过直接抑制脂质过氧化来抑制肠上皮细胞的氧化损伤。该结果可能阐明膳食芦丁在与红肉及加工肉类消费相关的结直肠癌发病预防中的作用。
