Jackson N, Ling N R, Ball J, Bromidge E, Nathan P D, Franklin I M
Department of Haematology, Medical School, Birmingham.
Clin Exp Immunol. 1988 Jun;72(3):351-6.
Fresh bone marrow from 43 cases of myeloma and three cases of plasma cell leukaemia has been phenotyped both by indirect immune-rosetting and, on fixed cytospin preparations, by indirect immunofluorescence. Both clustered and unclustered B cell associated antibodies from the IIIrd International Workshop on Human Leucocyte Differentiation Antigens were used. The results confirm the lack of many pan-B antigens on the surface of myeloma plasma cells, i.e. CD19-23, 37, 39, w40. Strong surface reactivity is seen with CD38 antibodies and with one CD24 antibody (HB8). Weak reactions are sometimes obtained with CD9, 10 and 45R. On cytospin preparations CD37, 39 and w40 are sometimes weakly positive, and anti-rough endoplasmic reticulum antibodies are always strongly positive. Specific and surface-reacting antiplasma cell antibodies are still lacking.
对43例骨髓瘤患者及3例浆细胞白血病患者的新鲜骨髓进行了表型分析，采用间接免疫玫瑰花结试验以及对固定的细胞涂片标本进行间接免疫荧光分析。使用了来自第三届人类白细胞分化抗原国际研讨会的聚集型和非聚集型B细胞相关抗体。结果证实骨髓瘤浆细胞表面缺乏许多泛B抗原，即CD19 - 23、37、39、w40。CD38抗体和一种CD24抗体（HB8）可见强表面反应性。CD9、10和45R有时会出现弱反应。在细胞涂片标本上，CD37、39和w40有时呈弱阳性，而抗粗面内质网抗体总是呈强阳性。目前仍缺乏特异性的表面反应性抗浆细胞抗体。
