Earnshaw W, Bordwell B, Marino C, Rothfield N
J Clin Invest. 1986 Feb;77(2):426-30. doi: 10.1172/JCI112320.
We have identified 39 individuals with anti-centromere antibodies (ACA) in our patient population, all of whom have Raynaud's syndrome or disease. We have used sera from the ACA-positive patients and from 123 controls (22 normal individuals and 101 additional patients with either Raynaud's disease or Raynaud's syndrome plus an associated connective tissue disease) to screen the proteins of highly purified human (HeLa) mitotic chromosomes by sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotting. Three antigens were recognized by the sera from the ACA-positive patients. These were centromere protein (CENP)-B (80,000 mol wt--recognized by all ACA-positive sera), CENP-A (17,000 mol wt--recognized by 38 of 39 ACA-positive sera), and CENP-C (140,000 mol wt--recognized by 37 of 39 ACA-positive sera). None of these antigens were recognized by any of the 123 control sera, although binding was occasionally seen to other chromosomal antigens. Therefore the ACA response is highly uniform in our patient population. Antibody to CENP-B shows a 100% correlation with anti-centromere staining by indirect immunofluorescence.
我们在患者群体中鉴定出39名抗着丝粒抗体（ACA）阳性个体，他们均患有雷诺综合征或雷诺病。我们使用ACA阳性患者的血清以及123名对照者（22名正常人以及101名患有雷诺病或雷诺综合征并伴有相关结缔组织病的患者）的血清，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳和免疫印迹法，对高度纯化的人（HeLa）有丝分裂染色体的蛋白质进行筛选。ACA阳性患者的血清识别出三种抗原。这些抗原分别是着丝粒蛋白（CENP）-B（分子量80,000——所有ACA阳性血清均可识别）、CENP-A（分子量17,000——39份ACA阳性血清中的38份可识别）和CENP-C（分子量140,000——39份ACA阳性血清中的37份可识别）。123份对照血清均未识别出这些抗原中的任何一种，尽管偶尔会观察到与其他染色体抗原的结合。因此，在我们的患者群体中，ACA反应高度一致。CENP-B抗体与间接免疫荧光法检测的抗着丝粒染色呈现100%的相关性。
