Cuesta-Rubio Osmany, Hernández Ingrid Márquez, Fernández Mercedes Campo, Rodríguez-Delgado Irán, De Oca Porto Rodny Montes, Piccinelli Anna Lisa, Celano Rita, Rastrelli Luca
Universidad Técnica de Machala, Facultad de Ciencias Químicas y de La Salud, Ave. Panamericana km 5½, 070101, Machala, Ecuador.
Universidad Técnica de Machala, Facultad de Ciencias Agropecurarias, Ave. Panamericana km 5½, 070101, Machala, Ecuador.
Phytochemistry. 2022 Nov;203:113415. doi: 10.1016/j.phytochem.2022.113415. Epub 2022 Aug 29.
The chemical composition and the antioxidant potential of Ecuadorian propolis samples (n = 19) collected in different provinces were investigated. HPLC-DAD-ESI/MS and GC-EI-MS analysis of the methanol extracts enabled us to define six types of Ecuadorian propolis based on their secondary metabolite composition. 68 compounds were identified, 59 of which are reported for the first time in Ecuadorian propolis. The detected compounds include flavonoids, diterpenes, triterpenes, organic acid derivatives, alkylresorcinol derivatives and nemorosone. Plants belonging to genera Populus, Mangifera and Clusia seemed to be vegetable sources employed by bees to produce Ecuadorian propolis. Total phenolic content and antioxidant activity of propolis extracts were determined by the Folin-Ciocalteu assay and 2,2-diphenyl-1-picrylhydrazyl and ferric reducing/antioxidant potential assays, respectively. As expected, the variable chemical composition affected the differences in terms of antioxidant potential.
对在厄瓜多尔不同省份采集的19份蜂胶样品的化学成分和抗氧化潜力进行了研究。对甲醇提取物进行的高效液相色谱-二极管阵列-电喷雾串联质谱（HPLC-DAD-ESI/MS）和气相色谱-电子轰击质谱（GC-EI-MS）分析，使我们能够根据其次生代谢产物组成确定六种类型的厄瓜多尔蜂胶。共鉴定出68种化合物，其中59种是首次在厄瓜多尔蜂胶中报道。检测到的化合物包括黄酮类、二萜类、三萜类、有机酸衍生物、烷基间苯二酚衍生物和蛇床子素。杨树属、芒果属和藤黄属植物似乎是蜜蜂用来生产厄瓜多尔蜂胶的植物来源。分别通过福林-酚试剂法、2,2-二苯基-1-苦基肼法和铁还原/抗氧化能力法测定了蜂胶提取物的总酚含量和抗氧化活性。正如预期的那样，化学成分的差异影响了抗氧化潜力的差异。
