Dipartimento di Farmacia, University of Salerno, Via Ponte Don Melillo, 84084 Fisciano, Italy.
J Agric Food Chem. 2013 May 29;61(21):5080-8. doi: 10.1021/jf400779w. Epub 2013 May 17.
Chemical composition of propolis samples from north Algeria was characterized by chromatographic and spectroscopic analyses. High-performance liquid chromatorgaphy with diode-array detection (HPLC-DAD) fingerprint of the methanol extracts allowed the definition of two main types of Algerian propolis (AP) directly related to their secondary metabolite composition. Investigation of two representative types of AP by preparative chromatographic procedure and mass spectrometric (MS) and NMR techniques led to the identification of their main constituents: caffeate esters and flavonoids from an AP type rich in phenolic compounds (PAP) and labdane and clerodane diterpenes, together with a polymethoxyflavonol, from an AP type containing mainly diterpenes (DAP). Subsequently, two specific HPLC-MS/MS methods for detection of PAP and DAP markers were developed to study the chemical composition of propolis samples of different north Algerian regions. Antioxidant activity of AP samples was evaluated by 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay, and a significant free-radical scavenging effect was observed for propolis of the PAP series rich in polyphenols.
采用色谱和光谱分析方法对来自阿尔及利亚北部的蜂胶样品的化学成分进行了表征。采用二极管阵列检测高效液相色谱法（HPLC-DAD）对甲醇提取物的指纹图谱进行分析，将两种主要类型的阿尔及利亚蜂胶（AP）直接与其次生代谢产物的组成相关联。通过制备色谱法和质谱（MS）和核磁共振（NMR）技术对两种代表性类型的 AP 进行研究，确定了它们的主要成分：富含酚类化合物的 AP 型中的咖啡酸酯和类黄酮（PAP），以及 labdane 和 clerodane 二萜类化合物，以及一种多甲氧基黄酮醇，主要含有二萜类化合物（DAP）。随后，开发了两种特定的 HPLC-MS/MS 方法来检测 PAP 和 DAP 标记物，以研究来自不同阿尔及利亚北部地区的蜂胶样品的化学成分。通过 2,2-二苯基-1-苦基肼基自由基（DPPH）测定法评估了 AP 样品的抗氧化活性，结果表明，富含多酚的 PAP 系列蜂胶具有显著的自由基清除作用。
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