Nguyen Doreen, Ivester Rachel G, Rosinke Kyle, Hoover Timothy R
Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA.
Molecules. 2025 Jan 7;30(2):204. doi: 10.3390/molecules30020204.
is a Gram-negative bacterium and human pathogen that is linked to various gastric diseases, including peptic ulcer disease, chronic gastritis, and gastric cancer. The filament of the flagellum is surrounded by a membranous sheath that is contiguous with the outer membrane. Proteomic analysis of isolated sheathed flagella from B128 identified the lipoprotein HP0135 as a potential component of the flagellar sheath. HP0135 is a small protein, with the mature HP0135 lipoprotein only 28 amino acid residues in length. Deletion of in B128 resulted in morphological abnormalities that included extensive formation of outer membrane vesicles and increased frequency of mini-cells. Introducing a plasmid-borne copy of into the Δ mutant suppressed the morphological abnormalities. The phenotype of the Δ mutant suggests HP0135 has roles in stabilizing the cell envelope and cell division.
是一种革兰氏阴性细菌和人类病原体，与多种胃部疾病有关，包括消化性溃疡病、慢性胃炎和胃癌。鞭毛的丝状体被一层与外膜相连的膜状鞘包围。对从B128分离出的带鞘鞭毛进行蛋白质组分析，确定脂蛋白HP0135是鞭毛鞘的潜在组成部分。HP0135是一种小蛋白，成熟的HP0135脂蛋白仅28个氨基酸残基。在B128中缺失导致形态异常，包括外膜囊泡的大量形成和小细胞频率增加。将携带的质粒拷贝引入Δ突变体可抑制形态异常。Δ突变体的表型表明HP0135在稳定细胞膜和细胞分裂中起作用。
