Lei Zhentian, Sumner Barbara W, Bhatia Anil, Sarma Saurav J, Sumner Lloyd W
University of Missouri Metabolomics Center, Columbia, Missouri.
Curr Protoc Plant Biol. 2019 Mar;4(1):e20085. doi: 10.1002/cppb.20085. Epub 2018 Nov 29.
Flavonoids are a class of specialized metabolites found in many different plant species. They protect against UV radiation, scavenge reactive oxygen species, are involved in plant defense responses, and are associated with plant-microorganism interactions. They have also been reported to possess health-promoting effects including anti-inflammatory, antioxidant, anticancer activity, and antihypertensive effects. Flavonoids encompass >10,000 structures where the types and amounts depend on the plant species, developmental stage, organ, and growth conditions. The diversity of flavonoid structures represents a significant challenge in the analysis of plant flavonoids. Many analytical techniques have been developed to detect and quantify flavonoids, and the most productive of these techniques use liquid chromatography (LC) coupled to mass spectrometry (MS) to analyze flavonoids due to the excellent combination of selectivity and sensitivity of MS. In addition, mass spectral libraries have been constructed to further aid flavonoid identification. Here, the use of ultra-high pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) in plant flavonoid analyses, with an emphasis on sample extraction, flavonoid separation, and MS detection, is described. © 2018 by John Wiley & Sons, Inc.
黄酮类化合物是一类存在于许多不同植物物种中的特殊代谢产物。它们能抵御紫外线辐射，清除活性氧，参与植物防御反应，并与植物 - 微生物相互作用有关。据报道，它们还具有促进健康的作用，包括抗炎、抗氧化、抗癌活性和降压作用。黄酮类化合物包含超过10000种结构，其类型和含量取决于植物物种、发育阶段、器官和生长条件。黄酮类化合物结构的多样性对植物黄酮类化合物的分析构成了重大挑战。已经开发了许多分析技术来检测和定量黄酮类化合物，其中最有效的技术是使用液相色谱（LC）与质谱（MS）联用，由于MS具有出色的选择性和灵敏度组合，因此可用于分析黄酮类化合物。此外，还构建了质谱图库以进一步辅助黄酮类化合物的鉴定。本文描述了超高压液相色谱与质谱联用（UHPLC-MS）在植物黄酮类化合物分析中的应用，重点介绍了样品提取、黄酮类化合物分离和MS检测。© 2018约翰威立父子出版公司
