Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Key Laboratory of Chemical Utilization of Plant Resources of Nanchang, College of Chemistry and Materials, Jiangxi Agricultural University, Nanchang 330045, China.
Hunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, China.
Molecules. 2022 Mar 14;27(6):1871. doi: 10.3390/molecules27061871.
A simple and label-free electrochemical aptasensor was developed for ultra-sensitive determination of chloramphenicol (CAP) based on a 2D transition of metal carbides (MXene) loaded with gold nanoparticles (AuNPs). The embedded AuNPs not only inhibit the aggregation of MXene sheets, but also improve the quantity of active sites and electronic conductivity. The aptamers (Apts) were able to immobilize on the MXene-AuNP modified electrode surface through Au-S interaction. Upon specifically binding with CAP with high affinity, the CAP-Apt complexes produced low conductivity on the aptasensor surface, leading to a decreased electrochemical signal. The resulting current change was quantitatively correlated with CAP concentration. Under optimized experimental conditions, the constructed aptasensor exhibited a good linear relationship within a wide range of 0.0001-10 nM and with a low detection limit of 0.03 pM for CAP. Moreover, the developed aptasensor has been applied to the determination of CAP concentration in honey samples with satisfactory results.
一种简单、无标记的电化学生物传感器，基于负载金纳米粒子（AuNPs）的二维过渡金属碳化物（MXene），用于超灵敏地测定氯霉素（CAP）。嵌入的 AuNPs 不仅抑制了 MXene 片的聚集，而且提高了活性位点的数量和电子电导率。适体（Apts）能够通过 Au-S 相互作用固定在 MXene-AuNP 修饰电极表面。与 CAP 特异性高亲和力结合后，CAP-Apt 复合物在适体传感器表面产生低电导率，导致电化学信号降低。所得电流变化与 CAP 浓度呈定量相关性。在优化的实验条件下，所构建的适体传感器在 0.0001-10 nM 的宽范围内表现出良好的线性关系，对 CAP 的检测限低至 0.03 pM。此外，该电化学生物传感器已成功应用于蜂蜜样品中 CAP 浓度的测定，结果令人满意。
