Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany.
Institute of Nutritional Medicine, University of Lübeck, Lübeck, Germany.
Biofactors. 2019 Mar;45(2):169-186. doi: 10.1002/biof.1467. Epub 2018 Nov 29.
The health and life span of Drosophila melanogaster are partly determined by intestinal barrier integrity, metabolic rate as well as stress response and the expression of longevity-associated genes, depending on genetic and dietary factors. Ursolic acid (UA) is a naturally occurring triterpenoid exhibiting potential antimicrobial, anti-inflammatory, and antiobesity activity and counteracting age-related deficits in muscle strength. In this study, UA was dietarily administered to w D. melanogaster which significantly elongated the health and life span of males. Spargel (srl) is the Drosophila orthologue of mammalian peroxisome proliferator-activated receptor-gamma coactivator 1 α(PGC1α), an important regulator of energy homeostasis and mitochondrial function. Our results indicate that the health-promoting effect of UA, demonstrated by a significant increase in climbing activity, occurs via an upregulation of srl expression leading to a metabolic shift in the fly without reducing fecundity or gut integrity. Moreover, UA affected the flies' microbiota in a manner that contributed to life span extension. Srl expression and microbiota both seem to be affected by UA, as we determined by using srl-mutant and axenic flies. © 2018 BioFactors, 45(2):169-186, 2019.
黑腹果蝇的健康和寿命部分取决于肠道屏障完整性、代谢率以及应激反应和与寿命相关基因的表达，这取决于遗传和饮食因素。熊果酸（UA）是一种天然存在的三萜，具有潜在的抗菌、抗炎和抗肥胖活性，并能抵抗与年龄相关的肌肉力量下降。在这项研究中，UA 通过饮食被给予 w D. melanogaster，这显著延长了雄性的健康和寿命。Spargel（srl）是果蝇与哺乳动物过氧化物酶体增殖物激活受体-γ共激活因子 1α（PGC1α）的同源物，PGC1α 是能量平衡和线粒体功能的重要调节剂。我们的结果表明，UA 通过上调 srl 表达来促进健康的作用，导致苍蝇发生代谢转变，而不会降低生育能力或肠道完整性。此外，UA 以一种有助于延长寿命的方式影响了苍蝇的微生物群。srl 表达和微生物群似乎都受到 UA 的影响，正如我们通过使用 srl 突变体和无菌苍蝇所确定的那样。生物因素，45（2）：169-186，2019。
