Department of Cardiology, Boai Hospital of Zhongshan, Zhongshan, 528403, China.
Department of Cardiology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510080, Guangdong, China.
Lipids Health Dis. 2018 Nov 30;17(1):272. doi: 10.1186/s12944-018-0921-3.
To investigate the effects of a Chinese herbal medicine Fufang-Zhenzhu Tiaozhi Capsule (FTZ) on restenosis and elucidate the mechanism of action.
A restenosis model was established by balloon rubbing the endothelium of the abdominal aorta followed by high fat diet. Rabbits were divided into blank control group, restenosis group, FTZ group (0.66 mg/kg/day), atorvastatin group (5 mg/kg/day) and FTZ + atorvastatin group (n = 8). Vascular stenosis was analyzed by X-ray. Serum levels of chemokines and cytokines interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-12 (IL-12), C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), monocyte chemoattractant protein-1 (MCP-1), and intercellular adhesion molecule-1 (ICAM-1) were measured by ELISA. The levels of NF-κB, IκB-α, P-IκBα, IKK-α, and P-IKKα/β from injured abdominal arteries were detected by Western blotting.
Restenosis was induced successfully via abdominal artery balloon injuries and high fat diet. Restenosis was significantly decreased in FTZ group compared with restenosis group (P < 0.05). FTZ group had markedly reduced serum lipid levels (P < 0.05). In addition, the levels of TNF-α, IL-1, IL-6, IL-8, IL-12, ICAM-1 and MCP-1 decreased by FTZ treatment (P < 0.05). The expression of NF-κB in the atherosclerotic lesions was significantly attenuated in FTZ group (P < 0.05).
FTZ could reduce restenosis via reducing NF-κB activity and inflammatory factor expression within the atherosclerotic lesion in a rabbit restenosis model. FTZ may be a new therapeutic agent for restenosis.
研究复方珍珠降脂胶囊（FTZ）对血管再狭窄的作用，并探讨其作用机制。
采用兔腹主动脉内皮球囊拉伤结合高脂饮食建立血管再狭窄模型，将实验动物分为空白对照组、模型组、FTZ 组（0.66mg/kg/d）、阿托伐他汀组（5mg/kg/d）和 FTZ+阿托伐他汀组（每组 8 只）。X 射线分析血管狭窄程度，ELISA 法检测血清趋化因子和细胞因子白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、白细胞介素-12（IL-12）、C 反应蛋白（CRP）、肿瘤坏死因子-α（TNF-α）、单核细胞趋化蛋白-1（MCP-1）和细胞间黏附分子-1（ICAM-1）的水平，Western blot 法检测损伤腹主动脉组织核因子-κB（NF-κB）、IκB-α、磷酸化 IκBα（P-IκBα）、IKK-α、磷酸化 IKKα/β（P-IKKα/β）的表达。
腹主动脉球囊拉伤联合高脂饮食成功诱导兔血管再狭窄模型，FTZ 组血管再狭窄程度较模型组明显减轻（P<0.05），FTZ 组血脂水平明显降低（P<0.05），同时 FTZ 组 TNF-α、IL-1、IL-6、IL-8、IL-12、ICAM-1 和 MCP-1 水平明显降低（P<0.05）。FTZ 组斑块组织 NF-κB 表达明显减弱（P<0.05）。
FTZ 可能通过抑制 NF-κB 活性和炎症因子表达减轻兔血管再狭窄，FTZ 可能是一种治疗血管再狭窄的新药物。
