Darbyshire Amanda K, Oliver Kendra H, Dupont William D, Plummer W Dale, Jones Carissa P, Boyd Kelli L
Division of Comparative Medicine, Vanderbilt University Medical Center, Nashville, Tennessee;, Email:
Division of Nephrology and Hypertension, Vanderbilt University Medical Center, Nashville, Tennessee.
J Am Assoc Lab Anim Sci. 2019 Jan 1;58(1):58-64. doi: 10.30802/AALAS-JAALAS-18-000040. Epub 2018 Nov 29.
Invertebrates are often overlooked as laboratory animals, yet they are commonly used in toxicology, developmental, cellular and molecular biology, and radiation studies with euthanasia as an endpoint. Little is known regarding appropriate euthanasia methods for invertebrate species, particularly for . Here, we evaluated the AVMA-recommended 2-step method of euthanasia in brine shrimp (). were exposed first to anesthetic solutions of 60% alcohol, 2.5 mg/L eugenol, or 4 g/L tricaine methanesulfonate (TMS) and then were transferred to euthanasia solutions of 70% alcohol, 95% alcohol, or 10% neutral buffered formalin. We examined time to anesthesia, behavioral response to anesthesia, anesthesia recovery, and time to euthanasia. Our results show that 2.5 mg/L eugenol and 4 g/L TMS inconsistently achieved anesthesia. Although 60% alcohol produced anesthesia, the time to anesthesia varied among replicate groups, and exposure resulted in an increase in abnormal behavior. We therefore do not recommend any of the tested anesthetic solutions for use in Although all 3 euthanasia solutions were effective, more research is needed to provide recommendations regarding euthanasia for this species.
无脊椎动物常被忽视作为实验动物，但它们常用于毒理学、发育生物学、细胞与分子生物学以及以安乐死为终点的辐射研究。对于无脊椎动物物种，尤其是[此处原文缺失具体物种信息]，合适的安乐死方法知之甚少。在此，我们评估了美国兽医协会（AVMA）推荐的对卤虫（[此处原文缺失具体物种信息]）进行安乐死的两步法。先将卤虫暴露于60%酒精、2.5毫克/升丁香酚或4克/升甲磺酸三卡因（TMS）的麻醉溶液中，然后转移至70%酒精、95%酒精或10%中性缓冲福尔马林的安乐死溶液中。我们检查了麻醉时间、对麻醉的行为反应、麻醉恢复情况以及安乐死时间。我们的结果表明，2.5毫克/升丁香酚和4克/升TMS不能始终如一地实现麻醉。虽然60%酒精能产生麻醉效果，但各重复组的麻醉时间有所不同，且暴露会导致异常行为增加。因此，我们不推荐将任何测试的麻醉溶液用于[此处原文缺失具体物种信息]。虽然所有3种安乐死溶液都有效，但仍需要更多研究来为该物种的安乐死提供建议。
